Pozdniakova A L
Biull Eksp Biol Med. 1987 Jan;103(1):3-5.
Application of antibodies to S-100 protein (the antibody concentration in the micropipette being 0.05 mg/ml) induced 13 +/- 4 mV depolarization of the membrane of snail Helix neurons. Non-immune gamma-globulin used in the control experiments caused no changes in the membrane potential. Antibody-induced depolarization was accompanied by a 2.5 nA inward current which was voltage-independent in the range of membrane potential from 50 to 110 mV. Hyperpolarization observed after the rinsing of antibodies was effectively blocked by a specific blocker of monovalent cation transport, ouabain, at a concentration of 5 X 10(-4) M. The absence of antibody-induced changes in the passive membrane conduction and the activation of electrogenic transport mechanisms after the antibody removal suggest possible involvement of Na, K- ATPase into the effect described.
应用抗S-100蛋白抗体（微量移液器中的抗体浓度为0.05mg/ml）可使蜗牛Helix神经元膜去极化13±4mV。对照实验中使用的非免疫γ球蛋白未引起膜电位变化。抗体诱导的去极化伴随着2.5nA的内向电流，该电流在膜电位50至110mV范围内与电压无关。冲洗抗体后观察到的超极化被浓度为5×10⁻⁴M的单价阳离子转运特异性阻滞剂哇巴因有效阻断。抗体去除后被动膜传导未出现抗体诱导的变化以及电生转运机制未被激活，这表明Na,K-ATP酶可能参与了上述效应。
