食物剥夺对大鼠福尔马林诱导的伤害性反应行为及β-内啡肽和性激素浓度的影响。

Effect of food deprivation on formalin-induced nociceptive behaviors and β-endorphin and sex hormone concentration in rats.

作者信息

Sarookhani Mohammad-Reza, Ghasemi-Dashkhasan Elmira, Heidari-Oranjaghi Nima, Azhdari-Zarmehri Hassan, Erami Elaheh, Hosseini Sedighe-Sadat

机构信息

Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.

Nursing and Midwifery School, Torbat Heydarieh University of Medical Sciences, Torbat Heydarieh, Iran.

出版信息

Iran Biomed J. 2014;18(2):107-13. doi: 10.6091/ibj.1212.2014.

DOI:10.6091/ibj.1212.2014

PMID:24518552

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3933920/

Abstract

BACKGROUND

The present study examined the possible role of endogenous opioidergic system in effect of food deprivation on formalin-induced nociceptive behaviors in male and female rats. Also, we investigated the effect of food deprivation on the plasma level of beta-endorphin and sex hormones.

METHODS

Food was withdrawn 48 h prior to performing the formalin test, but water continued to be available ad libitum. The formalin was injected into hind plantar paw.

RESULTS

There is significant difference between male and female control rats during phase 2B. Following 48-h food deprivation, both male and female rats exhibited enhanced nociceptive behavior in response to formalin. Food deprivation for 12 and 24 h increased and for 48 h decreased beta-endorphin level in male and female rats. Food deprivation for 24 h decreased testosterone level in male, while it had no significant effect on female rats and food deprivation for 48 h decreased testosterone level in both sexes. Food deprivation for 24 h increased estradiol level in female and that for 48 h had no significant effect on male and female rats.

CONCLUSIONS

The present study demonstrates the existence of food deprivation for 48 h causes enhancement of nociception in the formalin test in male and female rats that has correlation with decrease in plasma beta-endorphin and testosterone levels.

摘要

背景

本研究探讨了内源性阿片系统在食物剥夺对雄性和雌性大鼠福尔马林诱导的伤害性反应行为影响中的可能作用。此外，我们还研究了食物剥夺对血浆β-内啡肽和性激素水平的影响。

方法

在进行福尔马林试验前48小时停止喂食，但水可随意饮用。将福尔马林注射到后足底。

结果

在2B期，雄性和雌性对照大鼠之间存在显著差异。禁食48小时后，雄性和雌性大鼠对福尔马林的伤害性反应行为均增强。禁食12小时和24小时可增加雄性和雌性大鼠的β-内啡肽水平，禁食48小时则使其降低。禁食24小时可降低雄性大鼠的睾酮水平，而对雌性大鼠无显著影响；禁食48小时可降低两性的睾酮水平。禁食24小时可增加雌性大鼠的雌二醇水平，禁食48小时对雄性和雌性大鼠均无显著影响。