Laboratório de Neurociências, Departamento de Fisioterapia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São CarlosSP, Brasil, Laboratório de Neurociências, Departamento de Fisioterapia, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São Carlos, SP, Brasil.
Laboratório de Psicologia da Aprendizagem, Departamento de Psicologia, Centro de Educação e Ciências Humanas, Universidade Federal de São Carlos, São CarlosSP, Brasil, Laboratório de Psicologia da Aprendizagem, Departamento de Psicologia, Centro de Educação e Ciências Humanas, Universidade Federal de São Carlos, São Carlos, SP, Brasil.
Braz J Med Biol Res. 2014 Feb;47(2):135-43. doi: 10.1590/1414-431X20133429. Epub 2014 Jan 17.
This study investigated the effects of histamine H1 or H2 receptor antagonists on emotional memory consolidation in mice submitted to the elevated plus maze (EPM). The cerebellar vermis of male mice (Swiss albino) was implanted using a cannula guide. Three days after recovery, behavioral tests were performed in the EPM on 2 consecutive days (T1 and T2). Immediately after exposure to the EPM (T1), animals received a microinjection of saline (SAL) or the H1 antagonist chlorpheniramine (CPA; 0.016, 0.052, or 0.16 nmol/0.1 µL) in Experiment 1, and SAL or the H2 antagonist ranitidine (RA; 0.57, 2.85, or 5.7 nmol/0.1 µL) in Experiment 2. Twenty-four hours later, mice were reexposed to the EPM (T2) under the same experimental conditions but they did not receive any injection. Data were analyzed using one-way ANOVA and the Duncan test. In Experiment 1, mice microinjected with SAL and with CPA entered the open arms less often (%OAE) and spent less time in the open arms (%OAT) in T2, and there was no difference among groups. The results of Experiment 2 demonstrated that the values of %OAE and %OAT in T2 were lower compared to T1 for the groups that were microinjected with SAL and 2.85 nmol/0.1 µL RA. However, when animals were microinjected with 5.7 nmol/0.1 µL RA, they did not show a reduction in %OAE and %OAT. These results demonstrate that CPA did not affect behavior at the doses used in this study, while 5.7 nmol/0.1 µL RA induced impairment of memory consolidation in the EPM.
本研究探讨了组胺 H1 或 H2 受体拮抗剂对高架十字迷宫（EPM）中情绪记忆巩固的影响。雄性小鼠（瑞士白化）的小脑蚓部使用套管引导进行植入。恢复 3 天后，在连续 2 天（T1 和 T2）进行 EPM 行为测试。暴露于 EPM 后立即（T1），动物接受生理盐水（SAL）或 H1 拮抗剂氯苯那敏（CPA；0.016、0.052 或 0.16 nmol/0.1 µL）的微量注射，在实验 1 中，SAL 或 H2 拮抗剂雷尼替丁（RA；0.57、2.85 或 5.7 nmol/0.1 µL）在实验 2 中。24 小时后，在相同的实验条件下，将小鼠重新暴露于 EPM（T2），但不进行任何注射。使用单向方差分析和 Duncan 检验分析数据。在实验 1 中，SAL 和 CPA 微注射的小鼠在 T2 中较少进入开放臂（%OAE）和较少时间在开放臂（%OAT），且各组之间无差异。实验 2 的结果表明，与 T1 相比，SAL 和 2.85 nmol/0.1 µL RA 微注射组的 T2 中%OAE 和%OAT 值较低。然而，当动物被微注射 5.7 nmol/0.1 µL RA 时，它们在 %OAE 和 %OAT 中没有显示出减少。这些结果表明，CPA 在本研究中使用的剂量下不会影响行为，而 5.7 nmol/0.1 µL RA 诱导 EPM 中记忆巩固受损。
