Martinez M A, Marquez N, Ysern-Caldentey M
Depto. Biologia Celular, Univ. Simon Bolivar, Caracas, Venezuela.
Biochem Biophys Res Commun. 1988 Apr 15;152(1):30-6. doi: 10.1016/s0006-291x(88)80675-2.
The DNA and RNA concentrations in uninfected and Anaplasma marginale-infected bovine erythrocytes were determined. Bovine anaplasmosis was experimentally induced and the nucleic acid levels were followed during the development of the A. marginale infection. Two distinct growth stages were found, the "multiplication" stage and the "transfer" stage. Based on this hypothetycal developmental cycle, the amount of DNA and RNA per Anaplasma initial body was estimated as 73.1 x 10(-3) pg and 45.7 x 10(-3) pg respectively.
测定了未感染和感染边缘无形体的牛红细胞中的DNA和RNA浓度。通过实验诱导牛无形体病，并在边缘无形体感染发展过程中跟踪核酸水平。发现了两个不同的生长阶段，即“增殖”阶段和“转移”阶段。基于这个假设的发育周期，每个边缘无形体初始体的DNA和RNA量分别估计为73.1×10⁻³ pg和45.7×10⁻³ pg。
