Laboratory of Molecular Genetics of Bacteria, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague 14220, Czech Republic, Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Prague 12843, Czech Republic, Department of Structure Analysis of Biomacromolecules, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague 16206, Czech Republic, Laboratory of Structure and Function of Biomolecules, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague 14220, Czech Republic, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia and Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague 14220, Czech Republic.
Nucleic Acids Res. 2014 Apr;42(8):5151-63. doi: 10.1093/nar/gku113. Epub 2014 Feb 11.
Bacterial RNA polymerase (RNAP) is an essential multisubunit protein complex required for gene expression. Here, we characterize YvgS (HelD) from Bacillus subtilis, a novel binding partner of RNAP. We show that HelD interacts with RNAP-core between the secondary channel of RNAP and the alpha subunits. Importantly, we demonstrate that HelD stimulates transcription in an ATP-dependent manner by enhancing transcriptional cycling and elongation. We demonstrate that the stimulatory effect of HelD can be amplified by a small subunit of RNAP, delta. In vivo, HelD is not essential but it is required for timely adaptations of the cell to changing environment. In summary, this study establishes HelD as a valid component of the bacterial transcription machinery.
细菌 RNA 聚合酶（RNAP）是一种必需的多亚基蛋白复合物，是基因表达所必需的。在这里，我们对枯草芽孢杆菌中的 YvgS（HelD）进行了表征，它是 RNAP 的一个新的结合伴侣。我们表明 HelD 与 RNAP 核心在 RNAP 的次级通道和 α 亚基之间相互作用。重要的是，我们证明 HelD 通过增强转录循环和延伸以 ATP 依赖的方式刺激转录。我们证明，RNAP 的小亚基 delta 可以放大 HelD 的刺激作用。在体内，HelD 不是必需的，但它是细胞及时适应环境变化所必需的。总之，本研究确立了 HelD 是细菌转录机制的有效组成部分。
