Takeuchi T
Department of Obstetrics & Gynecology, Faculty of Medicine, University of Tokyo at Mejirodai, Japan.
Nihon Naibunpi Gakkai Zasshi. 1988 Nov 20;64(11):1124-39. doi: 10.1507/endocrine1927.64.11_1124.
It is known that amenorrhea, oligomenorrhea, irregular menstrual cycles, luteal insufficiency and infertility are frequently associated with hyperandrogenism. It has been reported in previous studies that the traditional herbal medicine, Shakuyaku-Kanzo-To(SKT) can lower high serum testosterone levels in oligomenorrheic or amenorrheic women, and that some of these sterile women conceive. SKT contains Shakuyaku (S) and Kanzo (K) in equal amounts. The main component of S and K is paeoniflorin and glycyrrhizin, respectively. This study was designed to investigate the mechanism in lowering serum testosterone (T) levels by SKT. Experiment I: Female Wistar rats were injected subcutaneously with 500 micrograms of testosterone propionate at the age of 2 days, becoming androgen-sterilized rats (ASR). Fifty-six-day-old ASR were given orally SKT (22.5, 45, 90 and 180 mg/kg body weight), S or K (11.25, 22.5, 45 and 90 mg/kg b.w.) in water through a tube every day for 2 weeks. Control ASR were given only water. Each group consisted of 10 rats. Serum total and free T levels in SKT and S groups were significantly lower than those in the controls, and these decreases were dose-dependent. Experiment II: Female Wistar rats were oophorectomized at the age of 60 days. From one week later they were given orally SKT (90 and 180 mg/kg b.w.), S or K (45 and 90 mg/kg b.w.) every day for 2 weeks. Control rats were given only water. Each group consisted of 11 rats. There was no change in serum T, LH and FSH levels in either groups. The results from experiment I and II suggest that SKT influences the T production by ovaries but not by adrenal glands. Experiment III: The minced tissues of one ovary obtained from proestrous Wistar rats were incubated with media containing Paeoniflorin(P), Glycyrrhetinic acid(GA) or Glycyrrhizin(GL) (GL) (1, 50 and 100 micrograms/ml, respectively, n = 5 in each group) for 270 minutes at 37 degrees C under an atmosphere of 95%O2 and 5%CO2. The T production by ovaries was significantly decreased in each treated group in comparison with the control, and this decrease was dose-dependent. However, the delta 4-androstenedione (delta 4-A) production by ovaries was increased in each treated group. The ratio of T to delta 4-A was significantly lower in each treated group than in the control. The estradiol(E2) production by ovaries in each treated group was not changed in comparison with the control.(ABSTRACT TRUNCATED AT 400 WORDS)
已知闭经、月经过少、月经周期不规律、黄体功能不全和不孕常与高雄激素血症相关。以往研究报道，传统草药芍药甘草汤（SKT）可降低月经过少或闭经女性的高血清睾酮水平，且部分不孕女性受孕。SKT中芍药（S）和甘草（K）含量相等。S和K的主要成分分别是芍药苷和甘草甜素。本研究旨在探讨SKT降低血清睾酮（T）水平的机制。实验I：2日龄雌性Wistar大鼠皮下注射500微克丙酸睾酮，成为雄激素不育大鼠（ASR）。56日龄的ASR每天经灌胃给予水中的SKT（22.5、45、90和180毫克/千克体重）、S或K（11.25、22.5、45和90毫克/千克体重），持续2周。对照ASR仅给予水。每组10只大鼠。SKT组和S组的血清总T和游离T水平显著低于对照组，且这些降低呈剂量依赖性。实验II：60日龄雌性Wistar大鼠行卵巢切除术。1周后，它们每天经灌胃给予SKT（90和180毫克/千克体重）、S或K（45和90毫克/千克体重），持续2周。对照大鼠仅给予水。每组11只大鼠。两组的血清T、LH和FSH水平均无变化。实验I和II的结果表明，SKT影响卵巢而非肾上腺产生T。实验III：将动情前期Wistar大鼠的一侧卵巢切碎组织与分别含芍药苷（P）、甘草次酸（GA）或甘草甜素（GL）（分别为1、50和100微克/毫升，每组n = 5）的培养基在37℃、95%O2和5%CO2的气氛中孵育270分钟。与对照组相比，各处理组卵巢产生的T均显著降低，且这种降低呈剂量依赖性。然而，各处理组卵巢产生的δ4 - 雄烯二酮（δ4 - A）增加。各处理组T与δ4 - A的比值显著低于对照组。各处理组卵巢产生的雌二醇（E2）与对照组相比无变化。（摘要截断于400字）
