Phillips D J, Wells T W, Reimer C B
Division of Host Factors, Centers for Disease Control, Atlanta, GA 30333.
Immunol Lett. 1988 Feb;17(2):159-68. doi: 10.1016/0165-2478(88)90085-5.
We measured the association constant (Ka) for 42 murine monoclonal antibodies (MAbs) to human IgG epitopes. Included are antibodies, previously evaluated in an IUIS/WHO collaborative study, to various epitopes on the four subclasses of human IgG - IgG Fc, IgG Fab, kappa, and lambda - and to selected IgG allotopes. We used a sequential-saturation immunofluorescent assay and interactive computer program to determine the Ka by Scatchard analysis. Kas ranged from unmeasurably low by this method (approximately 10(6) L/M) to 3.8 X 10(9) L/M. Some class specific MAbs had large Ka differences for different subclasses and some subclass specific MAbs had large Ka differences for molecules of the same subclass but of different light-chain types.
我们测定了42种鼠单克隆抗体（MAb）与人IgG表位的结合常数（Ka）。其中包括先前在国际免疫学会联盟/世界卫生组织合作研究中评估过的针对人IgG四个亚类（IgG Fc、IgG Fab、κ和λ）上各种表位以及选定IgG同种异型表位的抗体。我们使用顺序饱和免疫荧光测定法和交互式计算机程序，通过Scatchard分析来确定Ka。Ka范围从用此方法无法测量的低水平（约10⁶ L/M）到3.8×10⁹ L/M。一些类别特异性MAb对不同亚类有很大的Ka差异，一些亚类特异性MAb对同一亚类但不同轻链类型的分子有很大的Ka差异。
