Department of Vegetable Research, Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, 68 HaMaccabim Road, P.O. Box 15159, Rishon LeZion 7505101, Israel.
Department of Postharvest Science, Institute of Postharvest and Food Sciences, Agricultural Research Organization, Volcani Center, 68 HaMaccabim Road, P.O. Box 15159, Rishon LeZion 7505101, Israel.
Mol Plant Microbe Interact. 2020 Sep;33(9):1103-1107. doi: 10.1094/MPMI-05-20-0109-A. Epub 2020 Jul 28.
is a foliar necrotrophic fungal-pathogen capable of infecting >580 genera of plants, is often used as model organism for studying fungal-host interactions. We used RNAseq to study transcriptome of infection on a major (worldwide) vegetable crop, tomato (). Most previous works explored only few infection stages, using RNA extracted from entire leaf-organ diluting the expression of studied infected region. Many studied infection, on detached organs assuming that similar defense/physiological reactions occurs in the intact plant. We analyzed transcriptome of the pathogen and host in 5 infection stages of whole-plant leaves at the infection site. We supply high quality, pathogen-enriched gene count that facilitates future research of the molecular processes regulating the infection process.
是一种叶片性坏死真菌病原体,能够感染超过 580 属的植物,通常被用作研究真菌与宿主相互作用的模式生物。我们使用 RNA 测序研究了主要(全球范围内)蔬菜作物番茄()上的感染转录组。以前的大多数研究仅在少数几个感染阶段进行,使用从整个叶片器官中提取的 RNA 进行研究,从而稀释了受感染区域的表达。许多人研究了在离体器官上的感染,假设在完整植物中会发生类似的防御/生理反应。我们在感染部位的 5 个全株叶片感染阶段分析了病原体和宿主的转录组。我们提供高质量、病原体富集的基因计数,这将有助于未来研究调控感染过程的分子过程。