Willenborg D O, Parish C R
Neurosciences Research Unit, Royal Canberra Hospital, Australia.
J Immunol. 1988 May 15;140(10):3401-5.
A number of sulfated polysaccharides were tested for their ability to inhibit passively induced experimental allergic encephalomyelitis (EAE) in rats. Heparin and fucoidan both completely inhibited passive EAE even when treatment was begun 3 days after transfer of cells. Pentosan sulfate was partially inhibitory whereas chondroitin-4-sulfate had no effect. Inhibition was not merely due to killing of the cells since active sensitization 14 days after cell transfer resulted in an early onset of disease indicating the persistence of transferred cells as memory cells. Although all the inhibitory polysaccharides are anticoagulants, it would appear that this function alone is not the reason for inhibition since a heparin preparation devoid of anticoagulant activity also partially inhibited EAE. Actively induced EAE was also significantly delayed by treatment with heparin. The results are discussed in terms of the polysaccharides inhibiting the enzymatic dependent movement of lymphocytes across central nervous system vascular endothelium.
对多种硫酸化多糖进行了测试，以考察它们抑制大鼠被动诱导的实验性变应性脑脊髓炎（EAE）的能力。肝素和岩藻依聚糖即使在细胞转移3天后开始治疗，也都能完全抑制被动性EAE。硫酸戊聚糖具有部分抑制作用，而硫酸软骨素-4则没有效果。抑制作用不仅仅是由于细胞死亡，因为在细胞转移14天后进行主动致敏会导致疾病提前发作，这表明转移的细胞作为记忆细胞持续存在。尽管所有具有抑制作用的多糖都是抗凝剂，但似乎仅这一功能并非抑制的原因，因为一种没有抗凝活性的肝素制剂也能部分抑制EAE。用肝素治疗也能显著延迟主动诱导的EAE。根据多糖抑制淋巴细胞跨中枢神经系统血管内皮的酶依赖性运动对结果进行了讨论。
