Masterson W J, Taylor D, Turner M J
MRC Biochemical Parasitology Unit, Molteno Institute, University of Cambridge, United Kingdom.
J Immunol. 1988 May 1;140(9):3194-9.
A panel of variant-specific mAb has been raised against the Trypanosoma brucei variant MITat 1.2. The binding characteristics of these mAb have been determined by a combination of immunofluorescence assays, using living or fixed trypanosomes, and solid phase assays, using purified variant surface glycoprotein. In addition, these mAb have been tested for their ability to neutralize MITat 1.2 infections in mice. Finally, the epitopes recognized by the mAb have been defined by competitive binding assays. These results are discussed with respect to the structural organization of the surface coat of T. brucei.
已经制备了一组针对布氏锥虫变体MITat 1.2的变体特异性单克隆抗体。这些单克隆抗体的结合特性通过以下方法确定：结合使用活的或固定的锥虫的免疫荧光测定法，以及使用纯化的变体表面糖蛋白的固相测定法。此外，还测试了这些单克隆抗体中和小鼠中MITat 1.2感染的能力。最后，通过竞争性结合试验确定了单克隆抗体识别的表位。结合布氏锥虫表面被膜的结构组织对这些结果进行了讨论。
