Csako G, Suba E A, Tsai C M, Mocca L F, Elin R J
Clinical Pathology Department, National Institutes of Health, Bethesda, MD 20892.
J Clin Lab Immunol. 1987 Dec;24(4):193-8.
The antigenic properties of the highly purified US reference standard endotoxin (RSE) exposed to varying doses of ionizing radiation were studied with double immunodiffusion, immunoelectrophoresis and immunoblotting. Rabbit RSE antisera identified 2 distinct major antigenic components for untreated RSE: one related to the O-polysaccharide side chain ("O-antigenic specificity"), the other to the R-core. Based on a serologic cross-reactivity of R-core of RSE (Escherichia coli 0113) with the R-core of the lipopolysaccharide from E. coli 0111, the core type of E. coli 0113 was identified as coli R3. Increasing exposure of RSE to ionizing radiation progressively destroyed all antigenic reactivities: at lower doses of radiation the rate of elimination differed for the 2 antigen classes. The O-polysaccharide was more sensitive to gamma-radiation than the R-core and the O-antigenicity was lost before that of the R-core. Endotoxin molecules containing incomplete R-core (radiation-induced or mutant) did not react with the RSE antiserum.
采用双向免疫扩散、免疫电泳和免疫印迹法，研究了高纯度美国参考标准内毒素（RSE）在不同剂量电离辐射下的抗原特性。兔RSE抗血清鉴定出未处理的RSE有2种不同的主要抗原成分：一种与O-多糖侧链有关（“O抗原特异性”），另一种与R核心有关。基于RSE（大肠杆菌O113）的R核心与大肠杆菌O111脂多糖的R核心的血清学交叉反应，将大肠杆菌O113的核心类型鉴定为大肠杆菌R3。RSE暴露于电离辐射的剂量增加会逐渐破坏所有抗原反应性：在较低辐射剂量下，这2类抗原的消除速率不同。O-多糖比R核心对γ辐射更敏感，O抗原性在R核心之前丧失。含有不完全R核心（辐射诱导或突变）的内毒素分子不与RSE抗血清反应。
