Csako G, Elin R J, Hochstein H D, Tsai C M
Infect Immun. 1983 Jul;41(1):190-6. doi: 10.1128/iai.41.1.190-196.1983.
Techniques that reduce the toxicity of bacterial endotoxins are useful for studying the relationship between structure and biological activity. We used ionizing radiation to detoxify a highly refined endotoxin preparation. U.S. standard endotoxin EC. Dose-dependent changes occurred by exposure to 60Co-radiation in the physical properties and biological activities of the endotoxin. Sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis showed gradual loss of the polysaccharide components (O-side chain and R-core) from the endotoxin molecules. In contrast, although endotoxin revealed a complex absorption pattern in the UV range, radiation treatment failed to modify that pattern. Dose-related destruction of the primary toxic component, lipid A, was suggested by the results of activity tests: both the pyrogenicity and limulus reactivity of the endotoxin were destroyed by increasing doses of radiation. The results indicate that the detoxification is probably due to multiple effects of the ionizing radiation on bacterial lipopolysaccharides, and the action involves (i) the destruction of polysaccharide moieties and possibly (ii) the alteration of lipid A component of the endotoxin molecule.
降低细菌内毒素毒性的技术对于研究结构与生物活性之间的关系很有用。我们使用电离辐射对内毒素的高纯度制剂进行解毒。美国标准内毒素EC。通过暴露于60Co辐射，内毒素的物理性质和生物活性发生了剂量依赖性变化。十二烷基硫酸钠-聚丙烯酰胺平板凝胶电泳显示内毒素分子中的多糖成分（O侧链和R核心）逐渐丧失。相比之下，尽管内毒素在紫外范围内呈现出复杂的吸收模式，但辐射处理未能改变该模式。活性测试结果表明主要毒性成分脂质A存在剂量相关的破坏：内毒素的致热原性和鲎试剂反应性均因辐射剂量增加而被破坏。结果表明解毒可能是由于电离辐射对细菌脂多糖的多种作用，并且该作用涉及（i）多糖部分的破坏以及可能（ii）内毒素分子脂质A成分的改变。
