[Uptake and phosphorylation of exogenous substrates in Ankistrodesmus braunii : II. Effect of glycolic acid upon the incorporation of (32)P-labelled phosphate in dark and in light].

The findings of JACOBI (1959) that glycolate enhances the uptake and the incorporation of (32)P-labelled orthophosphate were re-investigated. In contrast to the findings of JACOBI we found that glycolic acid has no effect at concentrations between 10(-3) and 10(-7)M. The effect reported by JACOBI was seen only when instead of glycolic acid Na-glycolate was used in the experiments, as it was done by JACOBI. Further experiments showed that the enhancement of (32)P-incorporation is due only to the Na(+)-ions and not to glycolic acid. In addition it was found that up to pH 4,3 no glycolic acid is resorbed from the medium by cells of Ankistrodesmus braunii.