Exotic Animal Medicine Service, Alfort School of Veterinary Medicine, 7 Avenue du Général de Gaulle, Maisons-Alfort 94700, France.
Vet Rec. 2014 Mar 15;174(11):277. doi: 10.1136/vr.102127. Epub 2014 Feb 11.
Blood transfusion has been described in ferrets as a treatment for oestrus-associated anaemia and as a life-saving therapy following trauma, iatrogenic (usually surgery-induced) anaemia, autoimmune haemolytic anaemia and pure red cell aplasia. Although blood banking is a common method for storage of feline and canine blood it is not currently done with ferret blood. The aim of this study was to determine the shelf-life of ferret blood using the anticoagulant citrate-phosphate-dextrose-solution with adenine (CPDA). Two male ferrets were used as blood donors. From each ferret, 6 ml of blood was taken from the cranial vena cava and stored in 10 ml polyethylene terephthalate (PET) blood tubes containing 1 ml of CPDA solution. Blood was taken from each ferret once per month for five months. These 10 blood samples were stored in a laboratory refrigerator at 4°C for four weeks. Biochemical (glucose, pH, lactate, potassium, sodium) and haematological (haematocrit, light microscopic blood smear examination) analyses were performed on the stored blood at days 0, 7, 14, 21 and 28. Biochemical analyses revealed a progressive decrease from day seven in the stored blood pH, glucose and sodium, with a concomitant increase in lactate and potassium. These results are attributable to the ongoing metabolism and deterioration of the red blood cells (RBC) while in storage, and are more rapid than described for human or canine stored blood. Haematological analyses revealed a progressive elevation of the haematocrit due to the appearance of hypochromic red blood cells and echinocytes beginning at day 7. Haemolysis was observed in the microhaematocrit capillary tube sample by day 21, and microscopic clots were visible on the blood smear by day 28. The low blood pH and the appearance of many hypochromic RBCs and some echinocytes from day 7 in CPDA-stored ferret blood, suggest stored ferret blood has a short shelf-life when compared with stored human or canine blood. We recommend that ferret blood stored in CPDA should not be used for transfusion after seven days of storage at 4°C.
在雪貂中，输血已被描述为发情相关性贫血的治疗方法，以及创伤、医源性（通常是手术引起的）贫血、自身免疫性溶血性贫血和纯红细胞再生障碍后的救命疗法。虽然血库是储存猫科和犬科血液的常见方法，但目前还没有用雪貂血液进行储存。本研究的目的是使用含有腺嘌呤的柠檬酸-磷酸盐-葡萄糖-右旋糖（CPDA）来确定雪貂血液的保质期。使用 2 只雄性雪貂作为献血者。从每只雪貂的颈静脉采集 6 毫升血液，并储存在含有 1 毫升 CPDA 溶液的 10 毫升聚对苯二甲酸乙二醇酯（PET）血管中。每只雪貂每月采血一次，共进行五个月。这 10 个血样在实验室冰箱中于 4°C 储存 4 周。在储存的第 0、7、14、21 和 28 天，对血液进行生化（葡萄糖、pH 值、乳酸、钾、钠）和血液学（红细胞压积、血涂片光镜检查）分析。生化分析显示，储存血液的 pH 值、葡萄糖和钠从第 7 天开始逐渐下降，同时乳酸和钾升高。这些结果归因于红细胞（RBC）在储存过程中的持续代谢和恶化，比人类或犬科储存血液的描述更快。血液学分析显示，由于第 7 天开始出现低色素性红细胞和棘红细胞，红细胞压积逐渐升高。在第 21 天，微红细胞压积毛细管管样本中观察到溶血，在第 28 天，血涂片上可见显微镜下的血栓。CPDA 储存的雪貂血液从第 7 天开始出现低 pH 值和许多低色素性 RBC，以及一些棘红细胞，表明与储存的人类或犬科血液相比，储存的雪貂血液保质期较短。我们建议 CPDA 储存的雪貂血液在 4°C 储存 7 天后不应用于输血。
