Xie Xian-Fei, Wang Jian-Wu, Zhang Han-Ping, Li Qi-Xiong, Chen Bang-Yin
a Department of Pharmacology , Basic Medical School, Wuhan University , Wuhan 430072 , P.R. China.
Nat Prod Res. 2014;28(12):853-60. doi: 10.1080/14786419.2014.886208. Epub 2014 Feb 13.
Chemical composition, antimicrobial and antioxidant activities of the essential oil of Ampelopsis megalophylla were evaluated in this research. GC-MS analysis of the essential oil revealed 42 compounds, representing 88.54% of the oil. The major compounds were borneol (10.81%), α-pinene (6.74%) and β-elemene (6.23%). The antimicrobial activity of the essential oil was evaluated against 13 micro-organisms using the disc diffusion and broth microdilution methods. Results demonstrated higher effects of this oil against Gram-positive bacteria than the other reference strains tested. The antioxidant effect of the essential oil was evaluated by using 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,20-azinobis-3-ethylbenzthiazoline-6-sulfonate scavenging assays. The essential oil exhibited moderate antioxidant activity.
本研究对大叶蛇葡萄精油的化学成分、抗菌和抗氧化活性进行了评估。精油的气相色谱 - 质谱分析鉴定出42种化合物，占精油的88.54%。主要化合物为冰片（10.81%）、α-蒎烯（6.74%）和β-榄香烯（6.23%）。采用纸片扩散法和肉汤微量稀释法对该精油针对13种微生物的抗菌活性进行了评估。结果表明，该精油对革兰氏阳性菌的作用高于所测试的其他参考菌株。通过1,1 - 二苯基 - 2 - 苦基肼（DPPH）和2,2'-联氮 - 双 - 3 - 乙基苯并噻唑啉 - 6 - 磺酸清除试验评估了精油的抗氧化作用。该精油表现出中等程度的抗氧化活性。
