Chrysostomou Vicki, Kezic Jelena M, Trounce Ian A, Crowston Jonathan G
Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria, Australia.
Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
Neurobiol Aging. 2014 Jul;35(7):1722-5. doi: 10.1016/j.neurobiolaging.2014.01.019. Epub 2014 Jan 23.
We have previously shown that the optic nerve of mice becomes increasingly vulnerable to injury with advancing age. Here, we investigated whether regular exercise can modify this age-related vulnerability and improve optic nerve recovery after injury. Aged (12-month-old) C57BL/6J mice were exercised by swimming for 60 min/d, 5 d/wk for 6 weeks. After 5 weeks, injury to the optic nerve was induced by short-term elevation of intraocular pressure. Retinal function was recorded using the electroretinogram and the cellular and biochemical changes induced by injury were assessed using immunohistochemistry and quantitative polymerase chain reaction. We found that exercise almost completely reversed age-related vulnerability of the optic nerve to injury such that exercised aged mice had a similar functional response to injury as non-exercised young (3-month-old) mice. Exercise also abrogated injury-induced astrocytic gliosis and macrophage activation in the aged retina. These data suggest that the known benefits of exercise also extend to the visual system and support further investigation of physical activity as a means of protecting against injury, dysfunction, and degeneration in the aging eye.
我们之前已经表明，随着年龄的增长，小鼠的视神经对损伤变得越来越脆弱。在此，我们研究了规律运动是否可以改变这种与年龄相关的脆弱性，并改善损伤后视神经的恢复。对12月龄的老年C57BL/6J小鼠进行游泳锻炼，每天游泳60分钟，每周5天，持续6周。5周后，通过短期升高眼压诱导视神经损伤。使用视网膜电图记录视网膜功能，并使用免疫组织化学和定量聚合酶链反应评估损伤诱导的细胞和生化变化。我们发现，运动几乎完全逆转了与年龄相关的视神经对损伤的脆弱性，使得运动的老年小鼠对损伤的功能反应与未运动的年轻（3月龄）小鼠相似。运动还消除了老年视网膜中损伤诱导的星形胶质细胞增生和巨噬细胞活化。这些数据表明，运动的已知益处也扩展到视觉系统，并支持进一步研究体育活动作为一种预防衰老眼睛损伤、功能障碍和退化的手段。
