Kobayashi M
First Department of Surgery, Kochi Medical School, Nankoku, Japan.
Tohoku J Exp Med. 1988 Jan;154(1):71-89. doi: 10.1620/tjem.154.71.
We investigated the short-term effects of a single high-dose radiation upon transplanted MM46 tumor cells in mice by means of immunohistochemistry and electron microscopy. The irradiation induced: 1) giant cell formation from the 3rd day, 2) arrest of tumor cell mitosis in prophase and metaphase due to the disorganization of the mitotic spindles, 3) changes in immunoreactivity of laminin and cytoskeletons, and 4) multilayering of the vascular basal lamina and perivascular fibrosis. The above findings suggest a decrease in tumor cell compliance, growth and invasiveness and the potentiation of defensive host responses against vascular invasion after irradiation. The analysis of the temporal sequences of the events indicates that the time lapse between the optimal host response, tumor growth and invasion constitutes a critical period.
我们通过免疫组织化学和电子显微镜研究了单次高剂量辐射对小鼠移植MM46肿瘤细胞的短期影响。辐射诱导：1）从第3天开始形成巨细胞；2）由于有丝分裂纺锤体的紊乱，肿瘤细胞有丝分裂在前期和中期停滞；3）层粘连蛋白和细胞骨架免疫反应性的变化；4）血管基底膜多层化和血管周围纤维化。上述发现表明，辐射后肿瘤细胞的顺应性、生长和侵袭性降低，宿主对血管侵袭的防御反应增强。对这些事件时间顺序的分析表明，最佳宿主反应、肿瘤生长和侵袭之间的时间间隔构成了一个关键时期。
