Kobayashi Michiya, Furuya Yasuo, Okabayashi Takehiro, Araki Keijiro
Department of Tumor Surgery, Kochi Medical School, Oko-cho, Kohasu, Nankoku, 783-8505, Japan.
Med Mol Morphol. 2006 Jun;39(2):106-12. doi: 10.1007/s00795-005-0308-1.
The effect of single high-dose irradiation on collagen networks in tumor cells was examined by scanning electron microscopy. MM46 tumor cells were transplanted into C3H/He mice and locally irradiated with a 30 Gy dose (6 MeV) 7-8 days posttransplantation. Mice were killed at various time points postirradiation, and tumor tissues were excised and fixed. After 2 N NaOH maceration, tissues were washed and stained with 1% tannic acid. They were then postfixed with 1% OsO4, dehydrated, and dried. The tissues were coated with gold, and the three-dimensional structure of the collagen fibers was observed using scanning electron microscopy. In the control group, the chambers formed by collagen fibers were larger on days 7 and 14 than those on days 1 and 3. In the irradiated group, the chambers were largest and most irregular in shape on day 7. The chamber size had decreased approximately 50% by day 14, and there was evidence of tumor cell regeneration. In addition, several fenestrations in the chamber wall developed in the irradiated group. Fenestrations in the collagen chamber walls surrounding tumor cells might allow transfer of metabolites that provide nutrition to the tumor cells, leading to their regeneration.
通过扫描电子显微镜检查单次高剂量照射对肿瘤细胞中胶原网络的影响。将MM46肿瘤细胞移植到C3H/He小鼠体内，并在移植后7-8天对局部进行30 Gy剂量（6 MeV）的照射。在照射后的不同时间点处死小鼠，切除肿瘤组织并固定。经2N氢氧化钠浸渍后，洗涤组织并用1%单宁酸染色。然后用1%四氧化锇后固定，脱水并干燥。组织进行金镀膜，使用扫描电子显微镜观察胶原纤维的三维结构。在对照组中，由胶原纤维形成的腔室在第7天和第14天比第1天和第3天更大。在照射组中，腔室在第7天最大且形状最不规则。到第14天，腔室大小减少了约50%，并且有肿瘤细胞再生的迹象。此外，照射组腔室壁上出现了几个小孔。肿瘤细胞周围胶原腔室壁上的小孔可能允许为肿瘤细胞提供营养的代谢物转移，从而导致它们再生。
