Borase Hemant P, Patil Chandrashekhar D, Salunkhe Rahul B, Suryawanshi Rahul K, Salunke Bipinchandra K, Patil Satish V
School of Life Sciences, North Maharashtra University, Post Box 80, Jalgaon, 425001, Maharashtra, India.
Bioprocess Biosyst Eng. 2014 Aug;37(8):1695-705. doi: 10.1007/s00449-014-1142-4. Epub 2014 Feb 14.
Nowadays, increasing use of nanoproducts in area of human and environmental applications raises concern about safety aspects of nanoparticles synthesized using traditional physicochemical methods. Silver nanoparticles (AgNPs) synthesis at ambient parameters using latex of medicinally important plant Jatropha gossypifolia (J. gossypifolia) is reported in the present study. Potential of AgNPs in degradation of methylene blue and eosin B was also evaluated. Rapid formation of stable AgNPs was analyzed by visual color change from colorless to yellow-red after addition of latex in AgNO3 solution and by characteristic surface plasmon resonance (SPR) peak at 430 nm in UV-Vis spectroscopy. FT-IR analysis, protein coagulation test showed capping of proteins, flavonoids, terpenoids and polyphenols of latex on surface of AgNPs. FE-SEM, HR-TEM analysis revealed spherical shape of AgNPs. Narrow size range of AgNPs (5-40 nm) observed in HR-TEM analysis. EDS analysis confirms the presence of elemental silver while XRD revealed crystalline nature of AgNPs. Zeta potential of -21.4 mV indicates high stability of AgNPs. Effects of different parameters (pH, temperature, incubation time) on nanosynthesis were studied in the present study. Dye reduction studies were performed using UV-Vis spectroscopy, TLC, FT-IR and HPLC analysis showing decreased absorbance maxima of both dyes with respect to time, change in R f values, changes in wave number, transmittance, and retention time of dyes after AgNPs addition. The rate constant for methylene blue and eosin B reduction by AgNPs was found to be 0.062 and 0.022 min(-1).
如今，纳米产品在人类和环境应用领域的使用日益增加，这引发了人们对使用传统物理化学方法合成的纳米颗粒安全性的担忧。本研究报道了利用具有重要药用价值的植物麻风树（Jatropha gossypifolia）的乳胶在环境参数下合成银纳米颗粒（AgNPs）。还评估了AgNPs对亚甲基蓝和曙红B的降解潜力。通过在硝酸银溶液中加入乳胶后从无色到黄红色的视觉颜色变化以及紫外可见光谱中430 nm处的特征表面等离子体共振（SPR）峰，分析了稳定AgNPs的快速形成。傅里叶变换红外光谱（FT-IR）分析、蛋白质凝聚试验表明，乳胶中的蛋白质、黄酮类化合物、萜类化合物和多酚类物质包覆在AgNPs表面。场发射扫描电子显微镜（FE-SEM）、高分辨率透射电子显微镜（HR-TEM）分析显示AgNPs呈球形。在HR-TEM分析中观察到AgNPs的粒径范围较窄（5-40 nm）。能谱分析（EDS）证实了元素银的存在，而X射线衍射（XRD）显示了AgNPs的晶体性质。-21.4 mV的zeta电位表明AgNPs具有高稳定性。本研究考察了不同参数（pH值、温度、孵育时间）对纳米合成的影响。使用紫外可见光谱、薄层色谱（TLC）、傅里叶变换红外光谱和高效液相色谱（HPLC）分析进行染料还原研究，结果表明，加入AgNPs后，两种染料的最大吸光度随时间降低，Rf值、波数、透过率和染料保留时间发生变化。发现AgNPs还原亚甲基蓝和曙红B的速率常数分别为0.062和0.022 min-1。
