Cheynier R, Soulha M, Laure F, Vol J C, Reveil B, Gallo R C, Sarin P S, Zagury D
Université Pierre et Marie Curie, Paris, France.
AIDS Res Hum Retroviruses. 1988 Feb;4(1):43-50. doi: 10.1089/aid.1988.4.43.
Acquired immune deficiency syndrome (AIDS) is an immunosuppressive disease associated with the depletion of T4 lymphocytes. Recently, an HIV-1 genome was molecularly cloned and shown to be fully infectious in vitro by transfection experiments. In this study, we show that HIV-1 can be transfected into T4 and T8 lymphocyte subpopulations and viral proteins and infectious virus particles are observed in both short- and long-term cultures. In addition, transfected T8 cells can be maintained in culture for long periods without apparent cytopathic effect.
获得性免疫缺陷综合征（艾滋病）是一种与T4淋巴细胞耗竭相关的免疫抑制性疾病。最近，一种HIV-1基因组被分子克隆，并通过转染实验证明在体外具有完全传染性。在本研究中，我们表明HIV-1可转染至T4和T8淋巴细胞亚群，并且在短期和长期培养中均观察到病毒蛋白和感染性病毒颗粒。此外，转染的T8细胞可在培养中长期维持而无明显细胞病变效应。
