功能基因组学与系统生物学导论
An Introduction to Functional Genomics and Systems Biology.
作者信息
Bunnik Evelien M, Le Roch Karine G
机构信息
Department of Cell Biology and Neuroscience, University of California , Riverside, California.
出版信息
Adv Wound Care (New Rochelle). 2013 Nov;2(9):490-498. doi: 10.1089/wound.2012.0379.
OBJECTIVE
Over the past decade, the development of high-throughput technologies for DNA and protein analysis has revolutionized the ways in which cells can be studied. Within a relatively short time frame, research has changed from studying individual genes and proteins to analyzing entire genomes and proteomes.
APPROACH
In this article, we summarize the technologies and concepts that form the basis of this functional genomics approach.
RESULTS
Microarray and next-generation sequencing technologies have allowed researchers to investigate many different aspects of the cell, including DNA mutations, histone modifications, DNA methylation, chromatin structure, transcription, and translation on a genome-wide level. In addition, mass spectrometry technologies have undergone significant development and currently enable us to globally profile protein levels, protein-protein interactions, post-translational protein modifications, and metabolites.
INNOVATION AND CONCLUSION
The integration of information from the various processes that occur within a cell provides a more complete picture of how genes give rise to biological functions, and will ultimately help us to understand the biology of organisms, in both health and disease.
目的
在过去十年中，用于DNA和蛋白质分析的高通量技术的发展彻底改变了研究细胞的方式。在相对较短的时间内，研究已从研究单个基因和蛋白质转变为分析整个基因组和蛋白质组。
方法
在本文中，我们总结了构成这种功能基因组学方法基础的技术和概念。
结果
微阵列和下一代测序技术使研究人员能够在全基因组水平上研究细胞的许多不同方面，包括DNA突变、组蛋白修饰、DNA甲基化、染色质结构、转录和翻译。此外，质谱技术也取得了重大进展，目前使我们能够对蛋白质水平、蛋白质-蛋白质相互作用、蛋白质翻译后修饰和代谢物进行全局分析。
创新与结论
整合细胞内发生的各种过程的信息，可以更全面地了解基因如何产生生物学功能，并最终有助于我们理解生物体在健康和疾病状态下的生物学特性。
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