一项关于与互补决定区相关的人λ链表位的研究。
Studies of a human lambda-chain epitope related to a complementarity-determining region.
作者信息
Kim H S, Deutsch H F
机构信息
Department of Physiological Chemistry, University of Wisconsin, Madison 53706.
出版信息
Immunology. 1988 Apr;63(4):563-7.
A tryptic non-adecapeptide representing the 24-42 sequence of the MCG lambda-type Bence-Jones protein, and which contains its entire complementarity-determining region-1, was isolated. The peptide was utilized in preparing an affinity column that was used to isolate an antibody having the reactivity of a previously employed idiotypic antibody to MCG. This antibody preparation, as well as 13 monoclonal mouse antibodies to human lambda-chains, was employed in an enzyme-linked immunoassay to detect other Bence-Jones proteins with this serologic specificity. The results obtained with two of the monoclonal antibodies suggest that the epitope in question is a noncontiguous one.
分离出一种胰蛋白酶消化的非十九肽，它代表MCGλ型本斯-琼斯蛋白的24-42序列，并包含其完整的互补决定区-1。该肽用于制备亲和柱，该亲和柱用于分离具有先前使用的针对MCG的独特型抗体反应性的抗体。这种抗体制剂以及13种针对人λ链的单克隆小鼠抗体用于酶联免疫测定，以检测具有这种血清学特异性的其他本斯-琼斯蛋白。用两种单克隆抗体获得的结果表明，所讨论的表位是一个不连续的表位。
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