Etcheverrigaray M, Paladini A C, Retegui L A
Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Junín, Buenos Aires, Argentina.
Immunology. 1988 Apr;63(4):595-601.
Competition experiments between insolubilized monoclonal antibodies (mAb) and polyclonal antisera has led to the description of the humoral expression of human growth hormone (hGH) epitopes. This study was carried out with sera from mice and hamsters submitted to different immunization schedules: chronic administration of the antigen, secondary response and conventional hyperimmunization. The results indicated the absence of a unique immunodominant epitope in hGH; a significant individual variation of antibody (Ab) population titres with time; changes with time in the relative proportion of one Ab population with respect to the others; and the occurrence of Ab enhancing the 125I-hGH binding to five mAb depending upon the individuals and the time of immunization. Heterocliticity towards non-human GH was also detected. Although most of the animals showed cross-reacting Ab, two out of 12 mice, chronically injected, developed heteroclitic Ab. The data suggest that the humoral response to different epitopes of a protein antigen during the maturation of the immune response is a stochastic process leading to transient humoral immunodominance, enhancing Ab populations and heterocliticity, depending upon individual characteristics, either in outbred or inbred populations.
固定化单克隆抗体（mAb）与多克隆抗血清之间的竞争实验已得出关于人生长激素（hGH）表位体液表达的描述。本研究使用了接受不同免疫方案的小鼠和仓鼠血清：抗原的慢性给药、二次应答和传统的超免疫。结果表明，hGH中不存在独特的免疫显性表位；抗体（Ab）群体滴度随时间有显著的个体差异；一种Ab群体相对于其他群体的相对比例随时间变化；并且根据个体和免疫时间的不同，出现了增强125I-hGH与五种mAb结合的Ab。还检测到对非人生长激素的异嗜性。尽管大多数动物显示出交叉反应性Ab，但在12只慢性注射的小鼠中，有两只产生了异嗜性Ab。数据表明，在免疫反应成熟过程中，对蛋白质抗原不同表位的体液反应是一个随机过程，导致短暂的体液免疫显性、增强Ab群体和异嗜性，这取决于个体特征，无论是远交群体还是近交群体。
