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抗HLA - A2单克隆抗体的增强作用归因于抗体的二价性。

Enhancement of monoclonal antibodies against HLA-A2 is due to antibody bivalency.

作者信息

Holmes N J, Parham P

出版信息

J Biol Chem. 1983 Feb 10;258(3):1580-6.

PMID:6185487
Abstract

Enhancement between two monoclonal antibodies directed against the same antigen is when binding of one antibody, the enhancing antibody, increases the measured binding of the other antibody. This phenomenon has been observed for monoclonal antibodies against a variety of molecules including histocompatibility antigens. The mechanism of enhancement for monoclonal antibodies against HLA-A2 has been studied with purified preparations of IgG, F(ab')2 and Fab. Enhancement was only observed between antibodies against different antigenic sites. It requires bivalency of both antibody species and involves formation of stable, cyclic, tetramolecular complexes consisting of two antigen molecules and one each of the two antibody molecules. Conformational changes of either antigen or antibody do not appear to be important in this system, nor is the formation of combinatorial determinants between antigen and enhancing antibody. The enhancing properties of the antibodies studied are adequately explained in terms of their affinities and association and dissociation constants.

摘要

针对同一抗原的两种单克隆抗体之间的增强作用是指一种抗体（增强抗体）的结合会增加另一种抗体的测量结合。这种现象已在针对包括组织相容性抗原在内的多种分子的单克隆抗体中观察到。针对 HLA - A2 的单克隆抗体的增强机制已使用 IgG、F(ab')2 和 Fab 的纯化制剂进行了研究。仅在针对不同抗原位点的抗体之间观察到增强作用。它需要两种抗体都具有双价性，并且涉及由两个抗原分子和两个抗体分子各一个组成的稳定的环状四分子复合物的形成。在该系统中，抗原或抗体的构象变化似乎并不重要，抗原与增强抗体之间也不存在组合决定簇的形成。所研究抗体的增强特性可以根据它们的亲和力、缔合和解离常数得到充分解释。

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