Kandefer-Szerszeń M
Department of Applied Microbiology, Maria Curie-Sktodowska University, Lublin, Poland.
J Interferon Res. 1988 Feb;8(1):95-103. doi: 10.1089/jir.1988.8.95.
A comparative study of interferon (IFN) production (types alpha and gamma) was carried out using leukocytes from blood, spleen, and peritoneal cavity of sousliks (ground squirrels) active in summer, hibernating in winter, awakened from hibernation in winter, and hibernating in summer. Newcastle disease virus, Radom velogenic strain (NDV-R), and lipopolysaccharide from Escherichia coli (LPS) were used as inducers for IFN-alpha and phytohemagglutinin M (PHA) and concanavalin A (ConA) for IFN-gamma production. There were significant differences between the titers of IFN-alpha and IFN-gamma produced by leukocytes from sousliks hibernating in winter and in summer in comparison with titers of IFNs produced by cells of sousliks active in summer. Cells of hibernating spotted sousliks exhibited diminished IFN production. The IFN production in blood and peritoneal leukocytes of sousliks awakened from winter hibernation was also lower than that observed in cultures of leukocytes of sousliks active in summer, and higher when spleen leukocytes of sousliks awakened from hibernation were examined.
利用来自黄鼠（地松鼠）血液、脾脏和腹腔的白细胞，对夏季活跃、冬季冬眠、冬季从冬眠中苏醒以及夏季冬眠的黄鼠产生干扰素（IFN，包括α型和γ型）的情况进行了一项比较研究。新城疫病毒强毒株（NDV-R）和大肠杆菌脂多糖（LPS）用作诱导α干扰素产生的诱导剂，而植物血凝素M（PHA）和刀豆球蛋白A（ConA）用作诱导γ干扰素产生的诱导剂。与夏季活跃的黄鼠细胞产生的干扰素滴度相比，冬季和夏季冬眠的黄鼠白细胞产生的α干扰素和γ干扰素滴度存在显著差异。冬眠的花斑黄鼠细胞的干扰素产生减少。从冬季冬眠中苏醒的黄鼠血液和腹腔白细胞中的干扰素产生也低于夏季活跃的黄鼠白细胞培养物中的水平，而检查从冬眠中苏醒的黄鼠脾脏白细胞时，其干扰素产生水平更高。
