SNPSTR在存在基因座不相容的亲子鉴定中的效率案例研究。
A case study of SNPSTR efficiency in paternity testing with locus incompatibility.
作者信息
Ye Yi, Luo Haibo, Liao Linchuan, Zhang Ji, Wei Wei, Wang Zheng, Hou Yiping
机构信息
Department of Forensic Biology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China; Department of Forensic Analytical Toxicology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China.
Department of Forensic Biology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China.
出版信息
Forensic Sci Int Genet. 2014 Mar;9:72-5. doi: 10.1016/j.fsigen.2013.11.004. Epub 2013 Dec 4.
Instead of testing the additional STR loci, SNPSTR was included in the paternity testing for an alleged father-son duo case, with one inconsistency at the CSF1PO locus. We have chosen CSF1PO STR and five closely linked SNPs rs10077461, rs2569076, rs2228422, rs3733673 and rs3829987 to establish the SNPSTR and examined its potential use in paternity testing. A total of 152 haplotypes from 76 unrelated individuals were obtained by the nested-AS-PCR and 60 SNPSTR haplotypes were observed. The discrimination power of the SNPSTR haplotype was greater than either the STR or SNP haplotype alone. Its SNP part could be used to distinguish fathers from uncles. When SNPSTR was introduced into the calculation of parentage statistics, the paternity probability increased to 99.998%. Based on our findings, we concluded that SNPSTR could be considered a useful tool in forensic science.
在一个父子疑似案例的亲子鉴定中，未对额外的STR基因座进行检测，而是纳入了SNPSTR检测，结果在CSF1PO基因座发现一处不一致。我们选择了CSF1PO STR以及五个紧密连锁的单核苷酸多态性（SNP）rs10077461、rs2569076、rs2228422、rs3733673和rs3829987来构建SNPSTR，并研究其在亲子鉴定中的潜在用途。通过巢式等位基因特异性PCR（nested-AS-PCR）从76名无关个体中获得了总共152个单倍型，并观察到60种SNPSTR单倍型。SNPSTR单倍型的鉴别力大于单独的STR或SNP单倍型。其SNP部分可用于区分父亲和叔叔。当将SNPSTR纳入亲权统计计算时，父权概率提高到了99.998%。基于我们的研究结果，我们得出结论，SNPSTR可被视为法医学中的一种有用工具。
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