15个STR大麻基因座的特征分析：命名建议和SNPSTR单倍型
Characterization of 15 STR cannabis loci: nomenclature proposal and SNPSTR haplotypes.
作者信息
Valverde Laura, Lischka Christian, Scheiper Stefanie, Nedele Johanna, Challis Rachel, de Pancorbo Marian M, Pfeiffer Heidi, Köhnemann Stephan
机构信息
Institute of Legal Medicine, Münster University, Germany; BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU, Vitoria-Gasteiz, Spain.
Institute of Legal Medicine, Münster University, Germany.
出版信息
Forensic Sci Int Genet. 2014 Mar;9:61-5. doi: 10.1016/j.fsigen.2013.11.001. Epub 2013 Nov 19.
The standardization of methods for individualizing Cannabis sativa plants could offer new possibilities in the investigation of its illegal trade. Here we present the first nomenclature proposal for 15 cannabis STRs, which allows an initial standardization for performing comparisons between laboratories and generating genotype databases. Several alleles of the 15 STR loci have been sequenced. This has revealed that not all the STR loci are equally suitable for the individualization purposes. Moreover, several nucleotide variations have been detected both inside the repeat structure and/or in the flanking region. All the different SNPSTR haplotypes are presented and compared with the previous sequence raw data of the 15 STR loci. The SNPSTR data could considerably increase the informative value of the STRs, which could be very useful in complex cases.
大麻植株个体化方法的标准化可为其非法贸易调查提供新的可能性。在此，我们提出了15个大麻短串联重复序列（STR）的首个命名建议，这使得实验室间进行比较以及生成基因型数据库有了初步的标准化方法。已对这15个STR位点的多个等位基因进行了测序。结果表明，并非所有STR位点都同样适用于个体化目的。此外，在重复结构内部和/或侧翼区域均检测到了多个核苷酸变异。展示了所有不同的单核苷酸多态性短串联重复序列（SNPSTR）单倍型，并与之前15个STR位点的序列原始数据进行了比较。SNPSTR数据可显著提高STR的信息价值，这在复杂案件中可能非常有用。
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