Center for Biomedical Engineering and Regenerative Medicine, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
Center for Biomedical Engineering and Regenerative Medicine, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Department of Biologic and Materials Sciences, University of Michigan, 1011, North University Avenue, Room 2209, Ann Arbor, MI 48109, USA; Macromolecular Science and Engineering Center, University of Michigan, Ann Arbor, MI 48109, USA; Department of Materials Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Carbohydr Polym. 2014 Mar 15;103:110-8. doi: 10.1016/j.carbpol.2013.12.017. Epub 2013 Dec 16.
Cytocompatible injectable hydrogels with pH and temperature sensitivity based on carboxymethyl chitosan-graft-poly (N-isopropyl acrylamide)-glycidyl methacrylate (CMCS-PNIPAm-GMA) were prepared by UV crosslinking, and these hydrogels as localized drug carriers for anticancer drug and anti-inflammatory drug were also investigated. The chemical structure of CMCS-PNIPAm-GMA and of their hydrogels was characterized by FT-IR and NMR. The effect of PNIPAm grafting percentage, pH and temperature on the swelling ratio of the hydrogels was studied, demonstrating the pH/temperature-responsive nature of the hydrogels. The morphology of the hydrogels before and after swelling was observed by scanning electron microscope. 5-Fluorouracil and diclofenac sodium as model drugs were encapsulated into the hydrogels in situ. Moreover, the effect of pH and temperature on the release of these drugs was discussed. The cytocompatibility of the macromonomer CMCS-PNIPAm-GMA and their hydrogels was studied with dog bone marrow mesenchymal stem cells by using Alamar blue measurement and Live/Dead assay kit. All the results indicated that these degradable injectable hydrogels are good candidates for localized delivery systems of drugs.
基于羧甲基壳聚糖接枝聚（N-异丙基丙烯酰胺）-甲基丙烯酸缩水甘油酯（CMCS-PNIPAm-GMA）的具有 pH 和温度敏感性的细胞相容可注射水凝胶通过 UV 交联制备，并且这些水凝胶作为抗癌药物和抗炎药物的局部药物载体也进行了研究。CMCS-PNIPAm-GMA 及其水凝胶的化学结构通过 FT-IR 和 NMR 进行了表征。研究了 PNIPAm 接枝率、pH 和温度对水凝胶溶胀比的影响，表明了水凝胶的 pH/温度响应特性。通过扫描电子显微镜观察了水凝胶溶胀前后的形态。5-氟尿嘧啶和双氯芬酸钠作为模型药物被原位包封到水凝胶中。此外，还讨论了 pH 和温度对这些药物释放的影响。通过使用 Alamar blue 测量和 Live/Dead 测定试剂盒研究了犬骨髓间充质干细胞对大分子单体 CMCS-PNIPAm-GMA 及其水凝胶的细胞相容性。所有结果表明，这些可降解可注射水凝胶是药物局部递送系统的良好候选物。
