纤维素酶辅助提取蒲公英 Taraxacum officinale 多糖及其抑菌活性研究。
Cellulase-assisted extraction and antibacterial activity of polysaccharides from the dandelion Taraxacum officinale.
机构信息
School of Marine Science and Technology, Huaihai Institute of Technology, 59 Changwu Road, Lianyungang 222005, China.
出版信息
Carbohydr Polym. 2014 Mar 15;103:140-2. doi: 10.1016/j.carbpol.2013.12.029. Epub 2013 Dec 18.
In the present study, we investigated the cellulase-assisted extraction and antibacterial activity of water-soluble polysaccharides from the dandelion Taraxacum officinale. The extraction conditions, optimized for improving yield, were as follows: time, 46.11 min; temperature, 54.87 °C; pH, 4.51 and cellulase enzyme, 4000 U/g. Under these conditions, the yield of polysaccharides from dandelion (PD) reached 20.67% (w/w). The sugar content of PD was 95.6% (w/w), and it displayed high antibacterial activity at a concentration of 100mg/mL against Escherichia coli, Bacillus subtilis and Staphylococcus aureus. These results indicate that PD may be a viable option for use as a food preservative.
在本研究中，我们研究了蒲公英 Taraxacum officinale 中纤维素酶辅助提取和水溶性多糖的抗菌活性。为了提高产量，优化了提取条件，如下：时间，46.11 分钟；温度，54.87°C；pH 值，4.51 和纤维素酶酶，4000 U/g。在这些条件下，蒲公英（PD）多糖的产率达到 20.67%（w/w）。PD 的糖含量为 95.6%（w/w），在 100mg/mL 浓度下对大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌显示出高抗菌活性。这些结果表明 PD 可能是一种可行的食品防腐剂选择。
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