College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou, Zhejiang 310021, China.
Carbohydr Polym. 2014 Mar 15;103:434-41. doi: 10.1016/j.carbpol.2013.12.039. Epub 2014 Jan 8.
An oligosaccharide BBPW-2 was isolated and purified from Bombyx batryticatus, its molecular weight was 2.0 × 10(3)Da, and its structure was elucidated by compositional, methylation and NMR analysis. Our results showed that BBPW-2 consisted of β-D-(1 → 2,6)-glucopyranose and β-D-(1 → 2,6)-mannosyl units serving as the backbone, α-D-(1 → 2)-galactopyranose and α-D-(1 → 3)-mannosyl units as branches, and α-D-Manp and β-D-Glcp as terminals. The in vitro inhibitory activity of BBPW-2 was measured using MTT and and crystal violet assays, which suggested that BBPW-2 had direct cytotoxic effects on the cancer cell lines HeLa and HepG2 (particularly HeLa cells), and had a long-term antiproliferative effect on MCF-7 cells, respectively. Apoptosis and cellcycle analysis of HeLa cells showed that BBPW-2 induced cellcycle disruption in the G0/G1 and G2/M phases accompanied by an impressive increment of early apoptotic cells and late apoptotic and necrotic cells. These results suggest that BBPW-2 could be a potential chemotherapeutic drug and its antitumor effects deserve further study.
从家蚕中分离纯化得到一种寡糖 BBPW-2，其分子量为 2.0×10(3)Da，通过组成、甲基化和 NMR 分析阐明了其结构。结果表明，BBPW-2 由β-D-(1→2,6)-吡喃葡萄糖和β-D-(1→2,6)-甘露糖单元作为主链，α-D-(1→2)-吡喃半乳糖和α-D-(1→3)-甘露糖单元作为支链，以及α-D-Manp 和β-D-Glcp 作为末端。采用 MTT 和结晶紫法测定 BBPW-2 的体外抑制活性，结果表明 BBPW-2 对宫颈癌 HeLa 和肝癌 HepG2 细胞系（尤其是 HeLa 细胞）具有直接的细胞毒性作用，对 MCF-7 细胞具有长期的抗增殖作用。对 HeLa 细胞的细胞凋亡和细胞周期分析表明，BBPW-2 诱导细胞周期在 G0/G1 和 G2/M 期中断，同时早期凋亡细胞和晚期凋亡坏死细胞显著增加。这些结果表明，BBPW-2 可能是一种潜在的化疗药物，其抗肿瘤作用值得进一步研究。
