Xing Houjuan, Wang Zhilei, Wu Hongda, Zhao Xia, Liu Tao, Li Shu, Xu Shiwen
College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China; Animal Health Supervision Institute of Heilongjiang Province, 243 Haping Road, Xiangfang District, Harbin 150069, PR China.
Animal Health Supervision Institute of Heilongjiang Province, 243 Haping Road, Xiangfang District, Harbin 150069, PR China.
Ecotoxicol Environ Saf. 2015 Mar;113:491-8. doi: 10.1016/j.ecoenv.2014.12.040. Epub 2015 Jan 5.
This study assessed the impacts of atrazine (ATR), chlorpyrifos (CPF) and combined ATR/CPF exposure on the kidney of common carp (Cyprinus carpio L.). The carp were sampled after a 40-d exposure to CPF and ATR, individually or in combination, followed by a 40-d recovery to measure the expression levels of heat shock proteins genes (HSP60, HSP70 and HSP90) and pesticide residues in the kidney tissue. The results revealed that the mRNA and protein levels of HSP60, HSP70 and HSP90 were induced in the kidney of common carp by ATR, CPF, and ATR/CPF mixture. The accumulated amounts of ATR, CPF, and their metabolites in the kidney tissues exhibited dose-dependency. These results exhibited that increasing concentration of ATR and CPF in the environment causes considerable stress for common carp, suggesting that the expression levels of HSP60, HSP70 and HSP90 may act as potential biomarkers for assessing the environmental ATR and CPF risk for carp.
本研究评估了阿特拉津（ATR）、毒死蜱（CPF）以及ATR/CPF联合暴露对鲤鱼（Cyprinus carpio L.）肾脏的影响。鲤鱼在单独或联合暴露于CPF和ATR 40天后进行采样，随后经过40天的恢复，以测量热休克蛋白基因（HSP60、HSP70和HSP90）的表达水平以及肾脏组织中的农药残留。结果显示，ATR、CPF以及ATR/CPF混合物均可诱导鲤鱼肾脏中HSP60、HSP70和HSP90的mRNA和蛋白质水平升高。肾脏组织中ATR、CPF及其代谢产物的累积量呈现剂量依赖性。这些结果表明，环境中ATR和CPF浓度的增加会给鲤鱼带来相当大的压力，这表明HSP60、HSP70和HSP90的表达水平可能作为评估鲤鱼面临的环境ATR和CPF风险的潜在生物标志物。
