Naveed Muhammad Akram, Riaz Naheed, Saleem Muhammad, Jabeen Bushra, Ashraf Muhammad, Ismail Tayaba, Jabbar Abdul
Department of Chemistry, Baghdad-ul-Jadeed Campus, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
Department of Chemistry, Baghdad-ul-Jadeed Campus, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
Steroids. 2014 May;83:45-51. doi: 10.1016/j.steroids.2014.01.005. Epub 2014 Feb 12.
Longipetalosides A-C (1-3); three new furostane steroidal saponins together with (25S)-5α-furastan-3β,22,26-triol (4) and gitogenin (5) were isolated from the methanolic extract of the whole plant of Tribulus longipetalus. The structures of these compounds (1-5) were established by using 1D ((1)H, (13)C) and 2D NMR (HMQC, HMBC, COSY, NOESY) spectroscopy, and mass spectrometry (ESIMS, HRESIMS), and in comparison with literature data reported for related compounds. Compounds 1-5 were evaluated for their inhibitory activities against enzymes α-glucosidase, lipoxygenase, acetylcholinesterase, and butyrylcholinesterase. Only the compounds 4 and 5 were found as the inhibitors of enzyme α-glucosidase with IC50 values of 33.5±0.22 and 37.2±0.18μM, respectively.
长瓣蒺藜全株甲醇提取物中分离得到长瓣蒺藜苷A - C（1 - 3）；三种新的呋甾烷甾体皂苷，以及（25S）-5α-呋甾烷-3β，22，26 - 三醇（4）和吉托皂苷元（5）。通过一维（¹H，¹³C）和二维核磁共振（HMQC、HMBC、COSY、NOESY）光谱以及质谱（电喷雾离子化质谱、高分辨电喷雾离子化质谱），并与相关化合物的文献数据进行比较，确定了这些化合物（1 - 5）的结构。对化合物1 - 5进行了对α-葡萄糖苷酶、脂氧合酶、乙酰胆碱酯酶和丁酰胆碱酯酶的抑制活性评估。仅发现化合物4和5是α-葡萄糖苷酶的抑制剂，IC50值分别为33.5±0.22和37.2±0.18μM。
