National Resource Centre for Women's Health, Division for Women's and Children's Health, Oslo University Hospital, 0424 Oslo, Norway; Department of Dermatology, Oslo University Hospital, 0424 Oslo, Norway; Institute for Clinical Medicine, University of Oslo, Box 1171 Blindern, 0318 Oslo, Norway.
Department of Dermatology, Oslo University Hospital, 0424 Oslo, Norway; Institute for Clinical Medicine, University of Oslo, Box 1171 Blindern, 0318 Oslo, Norway.
Photodiagnosis Photodyn Ther. 2014 Jun;11(2):113-7. doi: 10.1016/j.pdpdt.2014.01.005. Epub 2014 Feb 14.
Genital erosive lichen planus (GELP) is a chronic inflammatory disease, in women characterized by painful vulvar and vaginal erosions. To prepare for a clinical trial on photodynamic treatment (PDT), we applied hexyl 5-aminolevulinate hydrochloride (HAL) in clinically normal and affected mucosa in 12 women with GELP using two different doses (6.25 or 50mg/ml). Biopsies were taken after 30 min and 3h. The biodistribution of HAL, measured as photoactive protoporphyrin IX (PpIX), was studied using non-invasive superficial fluorescence measurements and microscopic fluorescence photometry. More PpIX was detected after application of 12.5mg HAL than after 100mg, with large inter-individual variations. PpIX levels after 3h were overall higher than after 30 min. PpIX fluorescence was not detected in skin distant to the genital area. In conclusion, 6.25mg/ml HAL applied for 3h seems adequate for HAL absorption and conversion to PpIX in submucosal inflammatory and epithelial cells and can be used in a PDT trial of GELP.
生殖器侵蚀性扁平苔藓（GELP）是一种慢性炎症性疾病，其特征是女性外阴和阴道出现疼痛性溃疡。为了准备对光动力疗法（PDT）进行临床试验，我们在 12 名患有 GELP 的女性中，使用两种不同剂量（6.25 或 50mg/ml）的己基 5-氨基酮戊酸盐酸盐（HAL）在临床正常和受影响的粘膜上进行应用。在 30 分钟和 3 小时后进行活检。使用非侵入性浅表荧光测量和显微镜荧光光度法研究了 HAL 的生物分布，其测量方法为光活性原卟啉 IX（PpIX）。与应用 100mg 相比，应用 12.5mg HAL 后检测到更多的 PpIX，个体间差异较大。与 30 分钟后相比，3 小时后 PpIX 水平总体更高。在远离生殖器区域的皮肤中未检测到 PpIX 荧光。总之，应用 3 小时的 6.25mg/ml HAL 似乎足以吸收和将 PpIX 转化为粘膜下炎症和上皮细胞中的 PpIX，可用于 GELP 的 PDT 试验。
