原卟啉 IX 在局部应用六亚甲基乙二胺后女性生殖器腐蚀性扁平苔藓中的分布。
Biodistribution of protoporphyrin IX in female genital erosive lichen planus after topical application of hexaminolevulinate.
机构信息
National Resource Centre for Women's Health, Division for Women's and Children's Health, Oslo University Hospital, 0424 Oslo, Norway; Department of Dermatology, Oslo University Hospital, 0424 Oslo, Norway; Institute for Clinical Medicine, University of Oslo, Box 1171 Blindern, 0318 Oslo, Norway.
Department of Dermatology, Oslo University Hospital, 0424 Oslo, Norway; Institute for Clinical Medicine, University of Oslo, Box 1171 Blindern, 0318 Oslo, Norway.
出版信息
Photodiagnosis Photodyn Ther. 2014 Jun;11(2):113-7. doi: 10.1016/j.pdpdt.2014.01.005. Epub 2014 Feb 14.
Genital erosive lichen planus (GELP) is a chronic inflammatory disease, in women characterized by painful vulvar and vaginal erosions. To prepare for a clinical trial on photodynamic treatment (PDT), we applied hexyl 5-aminolevulinate hydrochloride (HAL) in clinically normal and affected mucosa in 12 women with GELP using two different doses (6.25 or 50mg/ml). Biopsies were taken after 30 min and 3h. The biodistribution of HAL, measured as photoactive protoporphyrin IX (PpIX), was studied using non-invasive superficial fluorescence measurements and microscopic fluorescence photometry. More PpIX was detected after application of 12.5mg HAL than after 100mg, with large inter-individual variations. PpIX levels after 3h were overall higher than after 30 min. PpIX fluorescence was not detected in skin distant to the genital area. In conclusion, 6.25mg/ml HAL applied for 3h seems adequate for HAL absorption and conversion to PpIX in submucosal inflammatory and epithelial cells and can be used in a PDT trial of GELP.
生殖器侵蚀性扁平苔藓（GELP）是一种慢性炎症性疾病，其特征是女性外阴和阴道出现疼痛性溃疡。为了准备对光动力疗法（PDT）进行临床试验，我们在 12 名患有 GELP 的女性中，使用两种不同剂量（6.25 或 50mg/ml）的己基 5-氨基酮戊酸盐酸盐（HAL）在临床正常和受影响的粘膜上进行应用。在 30 分钟和 3 小时后进行活检。使用非侵入性浅表荧光测量和显微镜荧光光度法研究了 HAL 的生物分布，其测量方法为光活性原卟啉 IX（PpIX）。与应用 100mg 相比，应用 12.5mg HAL 后检测到更多的 PpIX，个体间差异较大。与 30 分钟后相比，3 小时后 PpIX 水平总体更高。在远离生殖器区域的皮肤中未检测到 PpIX 荧光。总之，应用 3 小时的 6.25mg/ml HAL 似乎足以吸收和将 PpIX 转化为粘膜下炎症和上皮细胞中的 PpIX，可用于 GELP 的 PDT 试验。
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