Andreeva Nadezhda, Ryazanova Lubov, Dmitriev Vladimir, Kulakovskaya Tatiana, Kulaev Igor
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, Pushchino, Moscow Region, 142290, Russia.
Folia Microbiol (Praha). 2014 Sep;59(5):381-9. doi: 10.1007/s12223-014-0310-x. Epub 2014 Feb 16.
The basidiomycetous yeast Cryptococcus humicola was shown to be tolerant to manganese, cobalt, nickel, zinc, lanthanum, and cadmium cations at a concentration of 2.5 mmol/L, which is toxic for many yeasts. The basidiomycetous yeast Cryptococcus terreus was sensitive to all these ions and did not grow at the above concentration. In the presence of heavy metal cations, С. humicola, as opposed to C. terreus, was characterized by the higher content of acid-soluble inorganic polyphosphates. In vivo 4',6'-diamino-2-phenylindole dihydrochloride staining revealed polyphosphate accumulation in the cell wall and cytoplasmic inclusions of С. humicola in the presence of heavy metals. In C. terreus, polyphosphates in the presence of heavy metals accumulate mainly in vacuoles, which results in morphological changes in these organelles and, probably, disturbance of their function. The role of polyphosphate accumulation and cellular localization as factors of heavy metal tolerance of Cryptococcus humicola is discussed.
担子菌酵母土生隐球菌（Cryptococcus humicola）在浓度为2.5 mmol/L的锰、钴、镍、锌、镧和镉阳离子环境中表现出耐受性，而该浓度对许多酵母来说是有毒的。担子菌酵母地生隐球菌（Cryptococcus terreus）对所有这些离子敏感，在上述浓度下无法生长。在重金属阳离子存在的情况下，与地生隐球菌不同，土生隐球菌的特点是酸溶性无机多聚磷酸盐含量较高。体内4',6'-二氨基-2-苯基吲哚二盐酸盐染色显示，在重金属存在的情况下，土生隐球菌的细胞壁和细胞质内含物中有多聚磷酸盐积累。在地生隐球菌中，重金属存在时多聚磷酸盐主要积累在液泡中，这导致这些细胞器的形态变化，并可能扰乱其功能。文中讨论了多聚磷酸盐积累和细胞定位作为土生隐球菌对重金属耐受性因素的作用。
