Varsha Kontham Kulangara, Priya Sulochana, Devendra Leena, Nampoothiri Kesavan Madhavan
Biotechnology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum, 695 019, Kerala, India.
Appl Biochem Biotechnol. 2014 Apr;172(7):3402-13. doi: 10.1007/s12010-014-0779-4. Epub 2014 Feb 15.
Thirty-six lactic acid bacteria belong to Lactococcus, Lactobacillus, Enterococcus, and Pediococcus were isolated, and the spectrum of antifungal activity was verified against Fusarium oxysporum (KACC 42109), Aspergillus niger (KACC 42589), Fusarium moniliforme (KACC 08141), Penicillium chrysogenum (NII 08137), and the yeast Candida albicans (MTCC 3017). Three isolates, identified as Pediococcus pentosaceus (TG2), Lactobacillus casei (DY2), and Lactococcus (BSN) were selected further, and their antifungal compounds were identified by ESI-MS and HPLC analysis as a range of carboxylic acids along with some unidentified, higher molecular weight compounds. An attempt to check out the shelf life extension of wheat bread without fungal spoilage was performed by fermenting the dough with the Lactococcus isolate. Apart from growth in low pH and tolerance to bile salts, probiotic potential of these three isolates was further substantiated by in vitro screening methods that include transit tolerance to the conditions in the upper human gastrointestinal tract and bacterial adhesion capacity to human intestinal cell lines.
分离出36株属于乳球菌属、乳杆菌属、肠球菌属和片球菌属的乳酸菌，并针对尖孢镰刀菌（KACC 42109）、黑曲霉（KACC 42589）、串珠镰刀菌（KACC 08141）、产黄青霉（NII 08137）以及白色念珠菌（MTCC 3017）验证了其抗真菌活性谱。进一步挑选出三株鉴定为戊糖片球菌（TG2）、干酪乳杆菌（DY2）和乳球菌（BSN）的菌株，通过电喷雾电离质谱（ESI-MS）和高效液相色谱（HPLC）分析鉴定出它们的抗真菌化合物为一系列羧酸以及一些未鉴定的高分子量化合物。通过用乳球菌分离株发酵面团，尝试延长未受真菌污染的小麦面包的保质期。除了在低pH环境中生长以及对胆盐具有耐受性外，这三株分离株的益生菌潜力还通过体外筛选方法得到进一步证实，这些方法包括对人体上消化道环境的转运耐受性以及对人肠道细胞系的细菌黏附能力。
