Tamari Zvi, Rosin Dalia, Voichek Yoav, Barkai Naama
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
PLoS One. 2014 Feb 12;9(2):e88801. doi: 10.1371/journal.pone.0088801. eCollection 2014.
Cells adapt to environmental changes through genetic mutations that stabilize novel phenotypes. Often, this adaptation involves regulatory changes which modulate gene expression. In the budding yeast, ribosomal-related gene expression correlates with cell growth rate across different environments. To examine whether the same relationship between gene expression and growth rate is observed also across natural populations, we measured gene expression, growth rate and ethanol production of twenty-four wild type yeast strains originating from diverse habitats, grown on the pentose sugar xylulose. We found that expression of ribosome-related genes did not correlate with growth rate. Rather, growth rate was correlated with the expression of amino acid biosynthesis genes. Searching other databases, we observed a similar correlation between growth rate and amino-acid biosyntehsis genes in a library of gene deletions. We discuss the implications of our results for understanding how cells coordinate their translation capacity with available nutrient resources.
细胞通过稳定新表型的基因突变来适应环境变化。通常，这种适应涉及调节基因表达的调控变化。在出芽酵母中，核糖体相关基因的表达与不同环境下的细胞生长速率相关。为了研究在自然种群中是否也能观察到基因表达与生长速率之间的相同关系，我们测量了来自不同栖息地的24个野生型酵母菌株在戊糖木酮糖上生长时的基因表达、生长速率和乙醇产量。我们发现核糖体相关基因的表达与生长速率不相关。相反，生长速率与氨基酸生物合成基因的表达相关。在搜索其他数据库时，我们在一个基因缺失文库中观察到生长速率与氨基酸生物合成基因之间存在类似的相关性。我们讨论了我们的结果对于理解细胞如何将其翻译能力与可用营养资源相协调的意义。
