College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China; Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture, Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Food Res Int. 2024 Sep;191:114727. doi: 10.1016/j.foodres.2024.114727. Epub 2024 Jul 3.
Phages are a class of viruses that specifically infect host bacteria. Compared to other recognition elements, phages offer several advantages such as high specificity, easy to obtain and good environmental tolerance, etc. These advantages underscore the potential of phages as recognition elements in the construction of biosensors. Therefore, the phage-based biosensors are currently garnering widespread attention for detecting pathogens in recent years. However, the test performance such as detection limit, sensitivity and stability of exicting phage-based biosensors require enhancement. In the design of sensors, the selection of various materials and construction methods significantly influences the test performance of the sensor, and employing appropriate signal amplification strategies and construction methods to devise biosensors based on different principles is an effective strategy to enhance sensor performance. The manuscript primarily focuses on the signal amplification strategies and construction methods employed in phage-based biosensors recent ten years, and summarizes the advantages and disadvantages of different signal amplification strategies and construction methods. Meanwhile, the manuscript discusses the relationship between sensor performance and various materials and construction methods, and reviews the application progress of phage-based electrochemical biosensors in the detection of foodborne bacteria. Furthermore, the manuscript points out the present limitations and the future research direction for the field of phage-based biosensors, so as to provide the reference for developing high-performance phage-based biosensors.
噬菌体是一类专门感染宿主细菌的病毒。与其他识别元件相比，噬菌体具有特异性高、易于获取和良好的环境耐受性等优点。这些优点突出了噬菌体作为生物传感器构建中识别元件的潜力。因此，近年来，基于噬菌体的生物传感器因其能够检测病原体而受到广泛关注。然而，现有的基于噬菌体的生物传感器在检测限、灵敏度和稳定性等测试性能方面仍需提高。在传感器的设计中，各种材料的选择和构建方法对传感器的测试性能有显著影响，采用适当的信号放大策略和构建方法，基于不同原理设计生物传感器是提高传感器性能的有效策略。本文主要关注近十年来基于噬菌体的生物传感器中所采用的信号放大策略和构建方法，总结了不同信号放大策略和构建方法的优缺点。同时，本文讨论了传感器性能与各种材料和构建方法之间的关系，并综述了基于噬菌体的电化学生物传感器在食源性病原体检测方面的应用进展。此外，本文还指出了基于噬菌体的生物传感器领域目前存在的局限性和未来的研究方向，以期为开发高性能基于噬菌体的生物传感器提供参考。
