Laboratory for Cellular and Viral Oncogene Regulation, Carcinogenesis Research Institute, N, N, Blokhin Russian Cancer Research Center RAMS, 24, Kashirskoye sh,, Moscow 115478, Russia.
BMC Cancer. 2014 Feb 17;14:100. doi: 10.1186/1471-2407-14-100.
At the present time, there is a lack of data about the involvement of flotillins and stomatin in the development of non-small cell lung cancer (NSCLC) and soft tissue sarcomas (STS). Moreover, changes in expression of members of different families of the microdomain-forming proteins (caveolins and SPFH-domain containing family) are usually investigated independently of each other. In this study we performed a combined analysis of flotillins, stomatin, and caveolin-1 expression in these pathologies and evaluated correlations between generated data and clinicopathological characteristics of the specimens.
The protein and mRNA expression was analyzed by Western blotting and real-time PCR, respectively, in tissue specimens of patients undergoing surgery for non-small cell lung cancer and soft tissue sarcomas. Association between expression of studied proteins and patient clinicopathological characteristics or outcome was evaluated.
Stomatin protein expression was down-regulated in 80% of NSCLC samples and this decrease significantly associated with presence of lymph node metastases. Flotillin-2 protein expression was up-regulated in the majority of NSCLC samples whereas caveolin-1α expression was decreased. We revealed a strong correlation between STOM and FLOT-1 mRNA expression in both pathologies, although the gene expression changes were diverse.
Our data demonstrate for the first time that expression of stomatin, a poorly studied microdomain-forming protein, significantly changes in human tumors, thus pointing to its importance in the progression of NSCLC. We also suggest the existence of some relationship between the expression of these proteins.
目前，关于鞘糖脂和 stomatin 在非小细胞肺癌（NSCLC）和软组织肉瘤（STS）发展中的作用，相关数据十分缺乏。此外，不同微域形成蛋白家族（小窝蛋白和富含 SPFH 结构域家族）成员的表达变化通常是相互独立进行研究的。在这项研究中，我们对这些病理学中的鞘糖脂、stomatin 和 caveolin-1 的表达进行了联合分析，并评估了所产生数据与标本临床病理特征之间的相关性。
通过 Western blot 和实时 PCR 分别分析接受 NSCLC 和软组织肉瘤手术的患者组织标本中的蛋白和 mRNA 表达。评估研究蛋白的表达与患者临床病理特征或预后之间的关联。
80%的 NSCLC 样本中 stomatin 蛋白表达下调，且这种下调与淋巴结转移的存在显著相关。大多数 NSCLC 样本中 flotillin-2 蛋白表达上调，而 caveolin-1α 表达下调。我们在两种病理学中均发现 STOM 和 FLOT-1 mRNA 表达之间存在很强的相关性，尽管基因表达变化是多样的。
我们的数据首次表明，鞘糖脂是一种研究较少的微域形成蛋白，其在人类肿瘤中的表达显著改变，因此表明其在 NSCLC 进展中的重要性。我们还提示这些蛋白质的表达之间存在一定的关系。
