Moldovan Leni, Batte Kara E, Trgovcich Joanne, Wisler Jon, Marsh Clay B, Piper Melissa
Division of Pulmonary, Allergy, Critical Care, Sleep Medicine, College of Medicine, The Ohio State University, Columbus, OH, USA.
J Cell Mol Med. 2014 Mar;18(3):371-90. doi: 10.1111/jcmm.12236. Epub 2014 Feb 18.
MicroRNAs (miRNAs) have emerged as important regulators in the post-transcriptional control of gene expression. The discovery of their presence not only in tissues but also in extratissular fluids, including blood, urine and cerebro-spinal fluid, together with their changes in expression in various pathological conditions, has implicated these extracellular miRNAs as informative biomarkers of disease. However, exploiting miRNAs in this capacity requires methodological rigour. Here, we report several key procedural aspects of miRNA isolation from plasma and serum, as exemplified by research in cardiovascular and pulmonary diseases. We also highlight the advantages and disadvantages of various profiling methods to determine the expression levels of plasma- and serum-derived miRNAs. Attention to such methodological details is critical, as circulating miRNAs become diagnostic tools for various human diseases.
微小RNA（miRNA）已成为基因表达转录后调控中的重要调节因子。它们不仅存在于组织中，还存在于细胞外液中，包括血液、尿液和脑脊液，并且在各种病理状况下其表达会发生变化，这表明这些细胞外miRNA可作为疾病的信息性生物标志物。然而，要以这种能力利用miRNA需要严格的方法。在这里，我们报告了从血浆和血清中分离miRNA的几个关键程序方面，以心血管和肺部疾病的研究为例。我们还强调了各种分析方法用于确定血浆和血清来源的miRNA表达水平的优缺点。关注这些方法细节至关重要，因为循环miRNA正成为各种人类疾病的诊断工具。
