Villa S, Delia D
Bone Marrow Transplantation Unit, National Tumor Institute, Milan, Italy.
Exp Hematol. 1988 Jun;16(5):340-3.
Immunoenzymatic (IE) staining for antigen detection on intact human bone marrow (BM) colonies grown in agar layers is described. The reliability of this method allowed a study of antigenic changes during myelomonocytic differentiation. Phenotypic analysis was carried out at different times using the following monoclonal antibodies (MoAbs): S16.144, R1B19, UJ308, TG1 (CD 15), and UCHM1 (CD 14). The results confirmed that the clonogenic assay for human BM cells allows the detection of two granulo-macrophagic colony-forming cells (GM-CFC) appearing at different times during culture. In addition, this study pointed to a temporal sequence in the appearance of the markers analyzed: UJ308 seems the earliest detectable marker followed by R1B19, TG1, UCHM1, and S16.144, respectively. These findings suggest that the granulocytic differentiation occurs earlier than that of monocytes and that UJ308 is probably present on a subpopulation of myeloid cells expanded during hemopoietic regeneration.
本文描述了用于检测琼脂层中生长的完整人骨髓（BM）集落上抗原的免疫酶（IE）染色法。该方法的可靠性使得对髓单核细胞分化过程中的抗原变化进行研究成为可能。使用以下单克隆抗体（MoAbs）在不同时间进行表型分析：S16.144、R1B19、UJ308、TG1（CD 15）和UCHM1（CD 14）。结果证实，人BM细胞的克隆形成试验能够检测到培养过程中不同时间出现的两种粒-巨噬细胞集落形成细胞（GM-CFC）。此外，本研究指出了所分析标志物出现的时间顺序：UJ308似乎是最早可检测到的标志物，随后依次是R1B19、TG1、UCHM1和S16.144。这些发现表明粒细胞分化比单核细胞分化更早发生，并且UJ308可能存在于造血再生过程中扩增的髓细胞亚群上。
