Cancer Research UK Department of Medical Oncology, Glasgow University, Garscube Estate, Bearsden, Glasgow, U.K. (E-mail,
Cytotechnology. 2002 Jul;39(2):55-67. doi: 10.1023/A:1022949730029.
Cultured cell lines have become an extremely valuable resource, both in academic research and in industrial biotechnology. However, their value is frequently compromised by misidentification and undetected microbial contamination. As detailed elsewhere in this volume, the technology, both simple and sophisticated, is available to remedy the problems of misidentification and contamination, given the will to apply it. Combined with proper records of the origin and history of the cell line, assays for authentication and contamination contribute to the provenance of the cell line. Detailed records should start from the initiation or receipt of the cell line, and should incorporate data on the donor as well as the tissue from which the cell line was derived, should continue with details of maintenance, and include any accidental as well as deliberate deviations from normal maintenance. Records should also contain details of authentication and regular checks for contamination. With this information, preferably stored in a database, and suitable backed up, the provenance of the cell line so created makes the cell line a much more valuable resource, fit for validation in industrial applications and more likely to provide reproducible experimental results when disseminated for research in other laboratories.
细胞培养系已成为学术研究和工业生物技术中极其宝贵的资源。然而，它们的价值经常因错误鉴定和未检测到的微生物污染而受到损害。正如本卷其他地方详细介绍的那样，如果有应用该技术的意愿，那么纠正错误鉴定和污染问题的技术，无论是简单的还是复杂的，都可用。结合细胞系来源和历史的适当记录，鉴定和污染检测有助于证明细胞系的出处。详细的记录应从细胞系的建立或接收开始，并应包含供体以及细胞系来源组织的相关数据，应继续记录细胞系的维护细节，包括任何意外和故意偏离正常维护的情况。记录还应包含鉴定和定期污染检测的详细信息。有了这些信息，最好存储在数据库中，并进行适当备份，这样创建的细胞系的出处就使细胞系成为更有价值的资源，适合在工业应用中进行验证，并且在分发到其他实验室进行研究时更有可能提供可重复的实验结果。
