惠斯通电桥巨磁电阻细胞计数器。

Wheatstone bridge giant-magnetoresistance based cell counter.

机构信息

Department of Power Mechanical Engineering, National Tsing Hua University, Taiwan ROC.

Institute of Nanoengineering and Microsystems, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu 30013, Taiwan ROC.

出版信息

Biosens Bioelectron. 2014 Jul 15;57:48-53. doi: 10.1016/j.bios.2014.01.028. Epub 2014 Jan 25.

DOI:10.1016/j.bios.2014.01.028

PMID:24534580

Abstract

A Wheatstone bridge giant magnetoresistance (GMR) biosensor was proposed here for the detection and counting of magnetic cells. The biosensor was made of a top-pinned spin-valve layer structure, and it was integrated with a microchannel possessing the function of hydrodynamic focusing that allowed the cells to flow in series one by one and ensured the accuracy of detection. Through measuring the magnetoresistance variation caused by the stray field of the magnetic cells that flowed through the microchannel above the GMR biosensor, we can not only detect and count the cells but we can also recognize cells with different magnetic moments. In addition, a magnetic field gradient was applied for the separation of different cells into different channels.

摘要

本文提出了一种惠斯通电桥巨磁电阻（GMR）生物传感器，用于检测和计数磁性细胞。该生物传感器由顶层钉扎的自旋阀层结构组成，并与具有流体动力学聚焦功能的微通道集成，使细胞能够逐个串联流动，保证了检测的准确性。通过测量流过 GMR 生物传感器上方微通道的磁性细胞的杂散磁场引起的磁电阻变化，我们不仅可以检测和计数细胞，还可以识别具有不同磁矩的细胞。此外，还施加了磁场梯度，将不同的细胞分离到不同的通道中。