• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

评价血流式磁电阻芯片细胞计数器作为一种诊断工具。

Evaluation of In-Flow Magnetoresistive Chip Cell-Counter as a Diagnostic Tool.

机构信息

SPEC, CEA, CNRS, Université Paris-Saclay, CEA Saclay, CEDEX, 91191 Gif-sur-Yvette, France.

Service de Pharmacologie et Immunoanalyse (SPI), Laboratoire d'Etudes et de Recherches en Immunoanalyse, CEA, INRA, Université Paris-Saclay, 91191 Gif-sur-Yvette, France.

出版信息

Biosensors (Basel). 2019 Aug 31;9(3):105. doi: 10.3390/bios9030105.

DOI:10.3390/bios9030105
PMID:31480476
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6784370/
Abstract

Inexpensive simple medical devices allowing fast and reliable counting of whole cells are of interest for diagnosis and treatment monitoring. Magnetic-based labs on a chip are one of the possibilities currently studied to address this issue. Giant magnetoresistance (GMR) sensors offer both great sensitivity and device integrability with microfluidics and electronics. When used on a dynamic system, GMR-based biochips are able to detect magnetically labeled individual cells. In this article, a rigorous evaluation of the main characteristics of this magnetic medical device (specificity, sensitivity, time of use and variability) are presented and compared to those of both an ELISA test and a conventional flow cytometer, using an eukaryotic malignant cell line model in physiological conditions (NS1 murine cells in phosphate buffer saline). We describe a proof of specificity of a GMR sensor detection of magnetically labeled cells. The limit of detection of the actual system was shown to be similar to the ELISA one and 10 times higher than the cytometer one.

摘要

廉价的简单医疗设备可以快速可靠地计算全细胞，这对于诊断和治疗监测很有意义。基于磁性的芯片实验室是目前正在研究的解决这一问题的可能性之一。巨磁电阻（GMR）传感器具有高灵敏度和与微流控和电子设备的集成性。当应用于动态系统时，基于 GMR 的生物芯片能够检测到经磁标记的单个细胞。在本文中，对这种磁性医疗设备的主要特性（特异性、灵敏度、使用时间和可变性）进行了严格评估，并与 ELISA 测试和传统流式细胞仪进行了比较，使用生理条件下的真核恶性细胞系模型（磷酸盐缓冲盐水中的 NS1 鼠细胞）。我们描述了 GMR 传感器检测经磁标记细胞的特异性的证明。实际系统的检测限与 ELISA 相似，比细胞仪高 10 倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/5765896068b0/biosensors-09-00105-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/31f5c08bfb6b/biosensors-09-00105-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/8d7c5cccc3cf/biosensors-09-00105-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/97c3d97fa2a6/biosensors-09-00105-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/c7583ca144d7/biosensors-09-00105-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/d4a84eb274a3/biosensors-09-00105-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/6ad44af5fe6b/biosensors-09-00105-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/8111d06ab2ee/biosensors-09-00105-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/5765896068b0/biosensors-09-00105-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/31f5c08bfb6b/biosensors-09-00105-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/8d7c5cccc3cf/biosensors-09-00105-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/97c3d97fa2a6/biosensors-09-00105-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/c7583ca144d7/biosensors-09-00105-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/d4a84eb274a3/biosensors-09-00105-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/6ad44af5fe6b/biosensors-09-00105-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/8111d06ab2ee/biosensors-09-00105-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7540/6784370/5765896068b0/biosensors-09-00105-g007.jpg

相似文献

1
Evaluation of In-Flow Magnetoresistive Chip Cell-Counter as a Diagnostic Tool.评价血流式磁电阻芯片细胞计数器作为一种诊断工具。
Biosensors (Basel). 2019 Aug 31;9(3):105. doi: 10.3390/bios9030105.
2
The BARC biosensor applied to the detection of biological warfare agents.BARC生物传感器应用于生物战剂的检测。
Biosens Bioelectron. 2000 Jan;14(10-11):805-13. doi: 10.1016/s0956-5663(99)00054-8.
3
Evolution of a magnetic-based biomolecular detection system.基于磁性的生物分子检测系统的演变
Annu Int Conf IEEE Eng Med Biol Soc. 2009;2009:5425-7. doi: 10.1109/IEMBS.2009.5332482.
4
The Effect of Geometrical Overlap between Giant Magnetoresistance Sensor and Magnetic Flux Concentrators: A Novel Comb-Shaped Sensor for Improved Sensitivity.巨磁电阻传感器与磁通集中器之间几何重叠的影响：一种新型梳状传感器，可提高灵敏度。
Sensors (Basel). 2022 Dec 1;22(23):9385. doi: 10.3390/s22239385.
5
Magnetoresistive biosensors with on-chip pulsed excitation and magnetic correlated double sampling.带有片上脉冲激励和磁相关双采样的磁阻生物传感器。
Sci Rep. 2018 Nov 7;8(1):16493. doi: 10.1038/s41598-018-34720-0.
6
Giant Magnetoresistance Biosensors in Biomedical Applications.生物医学应用中的巨磁阻生物传感器
ACS Appl Mater Interfaces. 2022 Mar 2;14(8):9945-9969. doi: 10.1021/acsami.1c20141. Epub 2022 Feb 15.
7
GMR sensors: magnetoresistive behaviour optimization for biological detection by means of superparamagnetic nanoparticles.各向异性磁阻传感器：通过超顺磁纳米粒子优化用于生物检测的磁阻特性。
Biosens Bioelectron. 2011 Apr 15;26(8):3705-9. doi: 10.1016/j.bios.2011.02.013. Epub 2011 Feb 16.
8
Giant magenetoresistive sensors. 2. Detection of biorecognition events at self-referencing and magnetically tagged arrays.巨磁阻传感器。2. 自参考和磁性标记阵列中生物识别事件的检测。
Anal Chem. 2008 Nov 1;80(21):7940-6. doi: 10.1021/ac800967t. Epub 2008 Oct 1.
9
Magnetoresistive-based biosensors and biochips.基于磁阻的生物传感器和生物芯片。
Trends Biotechnol. 2004 Sep;22(9):455-62. doi: 10.1016/j.tibtech.2004.06.006.
10
A detection system based on giant magnetoresistive sensors and high-moment magnetic nanoparticles demonstrates zeptomole sensitivity: potential for personalized medicine.一种基于巨磁阻传感器和高矩磁性纳米颗粒的检测系统展现出了zeptomole灵敏度：对个性化医疗的潜力。
Angew Chem Int Ed Engl. 2009;48(15):2764-7. doi: 10.1002/anie.200806266.

引用本文的文献

1
Detection of pathogenic bacteria by magneto-immunoassays: a review.磁免疫分析检测病原菌：综述
J Biomed Res. 2020 Dec 25;35(4):277-283. doi: 10.7555/JBR.34.20200123.
2
Magneto-Impedance Biosensor Sensitivity: Effect and Enhancement.磁阻抗生物传感器灵敏度：影响与增强。
Sensors (Basel). 2020 Sep 12;20(18):5213. doi: 10.3390/s20185213.
3
Advances in Magnetoresistive Biosensors.磁阻生物传感器的进展。

本文引用的文献

1
An antibody targeting type III secretion system induces broad protection against Salmonella and Shigella infections.一种针对 III 型分泌系统的抗体可诱导针对沙门氏菌和志贺氏菌感染的广泛保护。
PLoS Negl Trop Dis. 2021 Mar 12;15(3):e0009231. doi: 10.1371/journal.pntd.0009231. eCollection 2021 Mar.
2
New advances in microfluidic flow cytometry.微流控流式细胞术的新进展
Electrophoresis. 2018 Sep 22. doi: 10.1002/elps.201800298.
3
Enhanced magnetic microcytometer with 3D flow focusing for cell enumeration.增强型磁微流控器，采用 3D 流聚焦技术，用于细胞计数。
Micromachines (Basel). 2019 Dec 26;11(1):34. doi: 10.3390/mi11010034.
Lab Chip. 2018 Aug 21;18(17):2593-2603. doi: 10.1039/c8lc00486b.
4
A sensitive and innovative detection method for rapid C-reactive proteins analysis based on a micro-fluxgate sensor system.基于微磁通门传感器系统的快速 C 反应蛋白分析的敏感创新检测方法。
PLoS One. 2018 Mar 30;13(3):e0194631. doi: 10.1371/journal.pone.0194631. eCollection 2018.
5
Hybrid integration of scalable mechanical and magnetophoretic focusing for magnetic flow cytometry.可扩展的机械和磁泳聚焦的混合集成用于磁流式细胞术。
Biosens Bioelectron. 2018 Jun 30;109:98-108. doi: 10.1016/j.bios.2018.02.046. Epub 2018 Mar 15.
6
Emerging Technologies for Molecular Diagnosis of Sepsis.脓毒症分子诊断的新兴技术。
Clin Microbiol Rev. 2018 Feb 28;31(2). doi: 10.1128/CMR.00089-17. Print 2018 Apr.
7
Magnetic biosensors: Modelling and simulation.磁生物传感器：建模与仿真。
Biosens Bioelectron. 2018 Apr 30;103:69-86. doi: 10.1016/j.bios.2017.12.023. Epub 2017 Dec 20.
8
Detection of BCG bacteria using a magnetoresistive biosensor: A step towards a fully electronic platform for tuberculosis point-of-care detection.利用磁阻生物传感器检测卡介苗细菌：迈向结核病即时检测全电子平台的一步。
Biosens Bioelectron. 2018 Feb 15;100:259-265. doi: 10.1016/j.bios.2017.09.004. Epub 2017 Sep 6.
9
Recent Advances in Magnetic Microfluidic Biosensors.磁性微流控生物传感器的最新进展
Nanomaterials (Basel). 2017 Jul 6;7(7):171. doi: 10.3390/nano7070171.
10
Sensors and bioassays powered by upconverting materials.上转换材料驱动的传感器和生物测定法。
Adv Colloid Interface Sci. 2017 Nov;249:66-87. doi: 10.1016/j.cis.2017.06.003. Epub 2017 Jun 10.