Huang T, Gong W H, Zou C P, Li X C, Jiang G J, Li X H, Qian H
Department of Orthopedics, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China
Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Genet Mol Res. 2014 Jan 21;13(1):354-62. doi: 10.4238/2014.January.21.3.
Marsdenia tenacissima extract (MTE) is a new plate-derived biotechnology product that is frequently used, but occasionally reported, in the field of chemotherapy. In this study, we assessed the antitumor activity and related mechanisms of MTE by various biotechnological methods. The survival rates of MG63 osteosarcoma cells treated with MTE and doxorubicin were measured, individually or jointly, and the changes in cellular shape, apoptotic rates, and Fas expression were observed. The results indicated that combination of MTE and doxorubicin up-regulated Fas expression and induced apoptosis. The survival rate of combined application of 50 mg/mL MTE and 1 μg/mL doxorubicin was significantly lower than that of the individual application (P < 0.01). Other biotechnology methods also showed an apoptosis-inducing effect of combined application that was much stronger than individual application. All of these results suggested that MTE may promote the effects of doxorubicin chemotherapy, perhaps related to the up-regulation of Fas expression in tumor cells.
通关藤提取物（MTE）是一种新的源自植物的生物技术产品，在化疗领域经常使用，但偶尔也有相关报道。在本研究中，我们通过各种生物技术方法评估了MTE的抗肿瘤活性及相关机制。分别或联合测量了用MTE和阿霉素处理的MG63骨肉瘤细胞的存活率，并观察了细胞形态、凋亡率和Fas表达的变化。结果表明，MTE与阿霉素联合使用可上调Fas表达并诱导凋亡。50mg/mL MTE与1μg/mL阿霉素联合应用的存活率显著低于单独应用（P<0.01）。其他生物技术方法也显示联合应用的诱导凋亡作用比单独应用强得多。所有这些结果表明，MTE可能促进阿霉素化疗的效果，这可能与肿瘤细胞中Fas表达上调有关。
