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A single 66-kilodalton polypeptide processed from the human immunodeficiency virus type 2 pol polyprotein in Escherichia coli displays reverse transcriptase activity.

作者信息

Le Grice S F, Zehnle R, Mous J

机构信息

Central Research Units, F. Hoffmann-LaRoche & Co. Ltd., Basel, Switzerland.

出版信息

J Virol. 1988 Jul;62(7):2525-9. doi: 10.1128/JVI.62.7.2525-2529.1988.

DOI:10.1128/JVI.62.7.2525-2529.1988
PMID:2453682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC253416/
Abstract

We have cloned the entire pol gene of human immunodeficiency virus type 2 into a high-level Escherichia coli expression system. Induction of cultures containing the recombinant plasmid, p2RTL1, leads to rapid accumulation of polypeptides of 66, 54, and 34 kilodaltons. We have designated the larger polypeptides reverse transcriptase, and we have designated the smaller polypeptide endonuclease. Purification of reverse transcriptase via ion-exchange and affinity chromatography yields the 66-kilodalton polypeptide, with which reverse transcriptase activity is associated. Purified enzyme furthermore displays a higher apparent molecular weight than its counterpart from human immunodeficiency virus type 1.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/499b/253416/0ddf6ba97410/jvirol00086-0327-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/499b/253416/d7382cf98095/jvirol00086-0326-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/499b/253416/a837b8016628/jvirol00086-0327-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/499b/253416/0ddf6ba97410/jvirol00086-0327-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/499b/253416/d7382cf98095/jvirol00086-0326-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/499b/253416/a837b8016628/jvirol00086-0327-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/499b/253416/0ddf6ba97410/jvirol00086-0327-b.jpg

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本文引用的文献

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