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感染重组痘苗病毒的细胞对人类免疫缺陷病毒1型gag和pol基因的表达及加工

Expression and processing of human immunodeficiency virus type 1 gag and pol genes by cells infected with a recombinant vaccinia virus.

作者信息

Gowda S D, Stein B S, Steimer K S, Engleman E G

机构信息

Department of Pathology, Stanford University School of Medicine, California 94305.

出版信息

J Virol. 1989 Mar;63(3):1451-4. doi: 10.1128/JVI.63.3.1451-1454.1989.

DOI:10.1128/JVI.63.3.1451-1454.1989
PMID:2464705
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC247850/
Abstract

Human cells infected with a recombinant vaccinia virus containing human immunodeficiency virus type 1 gag-pol genes produced large amounts of human immunodeficiency virus gag proteins beginning at 1 h and peaking at 48 h postinfection. We show that these polyproteins are processed accurately into mature forms and that the viral polymerase gene is encoded as a 160-kilodalton gag-pol fusion protein, most likely by translational frameshifting from the gag into the pol reading frame.

摘要

感染了含有1型人类免疫缺陷病毒gag-pol基因的重组痘苗病毒的人类细胞，在感染后1小时开始产生大量人类免疫缺陷病毒gag蛋白，并在48小时达到峰值。我们发现这些多聚蛋白被准确加工成成熟形式，并且病毒聚合酶基因被编码为一个160千道尔顿的gag-pol融合蛋白，很可能是通过从gag阅读框到pol阅读框的翻译移码产生的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0bb/247850/3c0edeeb1505/jvirol00070-0441-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0bb/247850/e5ed8f2f8a85/jvirol00070-0440-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0bb/247850/286436d85947/jvirol00070-0441-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0bb/247850/3c0edeeb1505/jvirol00070-0441-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0bb/247850/e5ed8f2f8a85/jvirol00070-0440-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0bb/247850/286436d85947/jvirol00070-0441-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0bb/247850/3c0edeeb1505/jvirol00070-0441-b.jpg

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