人乳头多瘤空泡病毒T抗原的比较。

J Gen Virol. 1976 Oct;33(1):61-70. doi: 10.1099/0022-1317-33-1-61.

A comparison was made of the T antigens induced in transformed cells or infected permissive cells by representatives of three categories of human papovavirus. The transformed hamster cell lines employed contained T antigen induced by either the BK or RF strains of papovavirus associated with human renal allografts; the JC strain of papovavirus from progressive multifocal leukoencephalopathy (PML), or a variant of SV40 virus isolated from PML. The human papovavirus T antigens were also compared with that of a human cell line transformed by SV40 of simian origin. Anti-T antibody prepared in hamsters against each of the hamster cell lines was absorbed with crude T antigen from each cell line, and the unabsorbed and absorbed antisera were tested for residual T antibody against each cell line, or against infected permissive cells by immunoperoxidase (IP) staining and complement-fixation (CF) tests. In unabsorbed antisera, T antibodies from each cell line cross-reacted with all T antigens in IP tests, and CF tests showed that T antisera reacted preferentially with T antigen induced by homologous virus. Absorpminants. T antigens of the two urine-derived strains, BK and RF, were identical or nearly so, but were clearly separable from T antigens of JC virus, PML-derived SV40 or simian-derived SV40. JC T antigen was intermediate, being more closely related to T antigens both of BK virus and SV40 virus than the latter were to each other. The T antigen of PML-derived SV40 could be distinguished from the T antigen of simian-derived SV40 and the T antigen of the SV40 variant from human brain was more closely related to those of the other human-derived papovaviruses than was the T antigen of SV40 from monkey kidney.

对三类人乳头多瘤病毒的代表毒株在转化细胞或感染的允许细胞中诱导产生的T抗原进行了比较。所使用的转化仓鼠细胞系含有由与人肾同种异体移植相关的乳头多瘤病毒BK株或RF株、来自进行性多灶性白质脑病（PML）的乳头多瘤病毒JC株或从PML分离的SV40病毒变体诱导产生的T抗原。还将人乳头多瘤病毒T抗原与由猿猴来源的SV40转化的人细胞系的T抗原进行了比较。用仓鼠针对每个仓鼠细胞系制备的抗T抗体用来自每个细胞系的粗制T抗原进行吸收，然后通过免疫过氧化物酶（IP）染色和补体结合（CF）试验，检测未吸收和吸收后的抗血清针对每个细胞系或感染的允许细胞的残余T抗体。在未吸收的抗血清中，每个细胞系的T抗体在IP试验中与所有T抗原发生交叉反应，CF试验表明T抗血清优先与同源病毒诱导产生的T抗原发生反应。吸收物。两种源自尿液的毒株BK和RF的T抗原相同或几乎相同，但与JC病毒、源自PML的SV40或源自猿猴的SV40的T抗原明显不同。JC T抗原处于中间位置，与BK病毒和SV40病毒的T抗原的关系比后两者之间的关系更密切。源自PML的SV40的T抗原可与源自猿猴的SV40的T抗原区分开来，并且源自人脑的SV40变体的T抗原与其他源自人的乳头多瘤病毒的T抗原的关系比源自猴肾的SV40的T抗原更密切。