Hénin Y, Maréchal V, Barré-Sinoussi F, Chermann J C, Morgenthaler J J
Viral Oncology Unit, Institut Pasteur, Paris, France.
Vox Sang. 1988;54(2):78-83. doi: 10.1111/j.1423-0410.1988.tb01621.x.
We studied the inactivation of the etiologic agent of the acquired immunodeficiency syndrome, human immunodeficiency virus, in the course of the Kistler and Nitschmann cold ethanol fractionation of human blood plasma. By measuring reverse transcriptase activity and viral infectivity, we have shown that the virus load is reduced by a factor of 10(4) during the initial and at least a factor of 10(6) during the subsequent steps of the fractionation procedure. This loss of virus may be observed in the absence or in the presence of antibodies against human immunodeficiency virus and is due to a combination of chemical inactivation, physical partition, and injury caused by repeated freezing and thawing. The laboratory data therefore further confirm epidemiological studies which indicate that immunoglobulin preparations obtained by ethanol fractionation do not transmit human immunodeficiency virus.
我们研究了在人血浆的基斯特勒和尼奇曼冷乙醇分级分离过程中获得性免疫缺陷综合征病原体——人类免疫缺陷病毒的失活情况。通过测量逆转录酶活性和病毒感染力，我们发现，在分级分离程序的初始步骤中病毒载量降低了10⁴倍，在后续步骤中至少降低了10⁶倍。无论有无抗人类免疫缺陷病毒抗体，均可观察到病毒的这种损失，这是化学失活、物理分配以及反复冻融造成的损伤共同作用的结果。因此，实验室数据进一步证实了流行病学研究的结果，即通过乙醇分级分离获得的免疫球蛋白制剂不会传播人类免疫缺陷病毒。
