Bolam J P, Izzo P N, Graybiel A M
MRC Anatomical Neuropharmacology Unit, Department of Pharmacology, Oxford, U.K.
Neuroscience. 1988 Mar;24(3):853-75. doi: 10.1016/0306-4522(88)90073-5.
In order to learn what morphological substrate might underly the histochemical compartments of the neostriatum, sections of the caudate nucleus and the putamen of cats and ferrets were stained immunocytochemically with antisera directed against several neuropeptides and transmitter-related enzymes and were then Golgi-impregnated. Adjacent sections were stained to reveal acetylcholinesterase activity to identify the acetylcholinesterase-poor striosomes. The immunostaining produced by several of the antibody preparations was in register with the acetylcholinesterase-poor striosomes but the most prominent staining of these zones occurred with the antibodies directed against substance P. The striosomes were delineated by intense substance P-immunostaining of neuronal perikarya and dendrites, and in the rostral and dorsal caudate nucleus the boundary between substance P-immunostained and extrastriosomal matrix was abrupt. For these reasons we analysed Golgi-impregnated neurons in sections immunostained for substance P in order to assess the influence of the chemically defined striosomal architecture on the position and dendritic arborization of neurons located both within the striosomes and within the extrastriosomal matrix. The most commonly impregnated neurons were of the medium-size densely spiny class. Those that were present within the striosomes and lay within one dendritic radius of the boundary were divided into two types: (1) neurons whose dendritic arborization was apparently not influenced by the boundary and (2) neurons whose dendritic arborization was markedly influenced by the boundary. For neurons of the latter type, dendrites either emerged from the parts of the perikaryon away from the boundary, so avoiding crossing it, or they exhibited abrupt changes in their course, apparently to avoid crossing the boundary. Spiny neurons located in the extrastriosomal matrix but close to the striosomal boundary had dendrites that were either influenced by, or not influenced by the compartmental boundary. We conclude that there is a specific cytoarchitecture underlying the histochemical compartments of the neostriatum and that different sub-populations of medium-size spiny neurons underly (1) the segregation of information flow in striosomes and the extrastriosomal matrix and (2) communication between striosomes and the extrastriosomal matrix.
为了了解何种形态学基质可能构成新纹状体组织化学分区的基础，我们用针对几种神经肽和与递质相关酶的抗血清对猫和雪貂的尾状核及壳核切片进行免疫细胞化学染色，然后进行高尔基染色。对相邻切片进行染色以显示乙酰胆碱酯酶活性，从而识别乙酰胆碱酯酶含量低的纹状体。几种抗体制剂产生的免疫染色与乙酰胆碱酯酶含量低的纹状体一致，但这些区域最显著的染色是由针对P物质的抗体产生的。纹状体由神经元胞体和树突的强烈P物质免疫染色勾勒出来，在尾状核的前部和背侧，P物质免疫染色区域与纹状体基质之间的边界是突然的。出于这些原因，我们分析了在P物质免疫染色切片中进行高尔基染色的神经元，以评估化学定义的纹状体结构对位于纹状体内和纹状体基质内的神经元的位置和树突分支的影响。最常被染色的神经元是中等大小的密集棘状神经元。那些位于纹状体内且位于边界一个树突半径范围内的神经元分为两类：（1）树突分支显然不受边界影响的神经元；（2）树突分支明显受边界影响的神经元。对于后一种类型的神经元，树突要么从远离边界的胞体部分发出，从而避免穿过边界，要么在其行程中表现出突然变化，显然是为了避免穿过边界。位于纹状体基质但靠近纹状体边界的棘状神经元的树突要么受分区边界影响，要么不受其影响。我们得出结论，新纹状体的组织化学分区存在特定的细胞结构，并且不同亚群的中等大小棘状神经元构成了（1）纹状体和纹状体基质中信息流的分离以及（2）纹状体与纹状体基质之间的通信的基础。
