Rusquet R M, Feon S A, David J C
Laboratoire de Biochimie du Développement, U.A. C.N.R.S. No. 256, Université de Rennes I, France.
Cancer Res. 1988 Jul 15;48(14):4038-44.
DNA ligase activities were determined in blood samples of 431 different cases of lymphoblastic and nonlymphoblastic leukemia. Less activity was observed in samples from lymphoblastic patients. RNA translation together with ligase immunoprecipitation experiments show that in T-cell acute lymphoblastic leukemic cells, no ligase is translated. This deficiency is correlated with the presence of more breaks in the DNA from these kinds of leukemia which results in altered DNA. This DNA can be ligated by the addition of exogenous ligase. This is the first demonstration of a ligase deficiency in leukemic human cells. These results are discussed in terms of chromosome abnormalities and rearrangements of genes coding for enzymes involved in DNA replication and repair.
在431例不同类型的淋巴细胞性和非淋巴细胞性白血病患者的血样中测定了DNA连接酶活性。在淋巴细胞性白血病患者的样本中观察到活性较低。RNA翻译以及连接酶免疫沉淀实验表明，在T细胞急性淋巴细胞白血病细胞中，没有连接酶被翻译。这种缺陷与这类白血病的DNA中存在更多断裂相关，这导致DNA发生改变。通过添加外源性连接酶可以连接这种DNA。这是首次在人类白血病细胞中证明连接酶缺陷。从染色体异常以及编码参与DNA复制和修复的酶的基因重排方面对这些结果进行了讨论。
